The objective of this study was to determine the risk of clinical mastitis in the first 120 d in lactation based on previous somatic cell count (SCC) history in a herd with a very low prevalence of contagious pathogens. A total of 218 cows from a university herd were enrolled at dry-off. Duplicate quarter milk samples were collected from all quarters at dry-off, postcalving (2 to 9 d in milk), ...

Vaccination can play a useful role in mastitis control programs, although there is a relative dearth of large, well-controlled field efficacy studies. This paper presents the findings on the use of a commercially available vaccine (Startvac, Hipra UK Ltd., Nottingham, UK) on commercial units under UK field conditions. In total, 3,130 cows were recruited from 7 farms and were randomly allocated,...

Mastitis is the most common and economically significant disease affecting dairy cattle. A variety of bacteria can be isolated from bovine mastitis cases. and are the most common causes of contagious and environmental clinical mastitis, respectively. Antimicrobial therapy is commonly implemented for mastitis prevention and control. Unfortunately, despite the best possible antimicrobial treatmen...

In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF- α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with a...

Factors related to the distribution of clinical bovine mastitis between rear and front quarters were studied using data from a 4 year survey of commercial dairy herds in western France. The study involved 844 mastitis cases affecting 597 lactations of 500 French Friesian cows from 44 herds. Risk factor hypotheses were related to certain aspects of lactation, udder conformation and management pr...

Amplicon sequencing technique has been increasingly applied to the clinical setting as a sensitive diagnostic tool. Therefore, it is of great importance to develop a DNA extraction method that accurate isolates DNA from complex host-associated microbiota. Given the multifactorial etiology of clinical mastitis and the diversified lifestyle of bacterial species harboring in milk, here four distin...

Fecal contamination of bedding has been associated with coliform mastitis caused by E. coli, while coliform mastitis caused by Klebsiella spp. is associated with wet sawdust and wood shavings.(1) Coliform mastitis has an average incidence rate of 15% to 20% in herds with low somatic cell counts (SCC) and is implicated in 30% to 40% of clinical cases. It is a common cause of fatal mastitis in ca...

The indiscriminate and injudicious administration of antibiotics and irrational treatment of bovine mastitis with different antibiotics have invited serious complications like multiple drug resistance. The present paper reports the microbiological examination and reporting of a clinical case of mastitis in cow and its recommended therapeutic management with the selected broad spectrum antibioti...

Mastitis is by far the most common and most costly disease in milk production in industrialised countries. Costs are related to loss in milk quality, decreased milk production related to subclinical mastitis, discarded milk, replacement cows, cost of treatment and labour costs [1]. This disease is the most frequent reason for the use of antimicrobials in dairy cows [2]. It can be classified int...

The objective of this multi-state, multi-herd clinical trial was to report on the efficacy of using an on-farm culture system to guide strategic treatment decisions in cows with clinical mastitis. The study was conducted in 8 commercial dairy farms ranging in size from 144 to 1,795 cows from Minnesota, Wisconsin, and Ontario, Canada. A total of 422 cows affected with mild or moderate clinical m...