The anion channel protein from Clavibacter michiganense ssp. nebraskense (Schürholz, Th. et al. 1991, J. Membrane Biol. 123: 1-8) was analyzed at different concentrations of KCl and KF. At 0.8 M KCl the conductance G(V(m)) increases exponentially from 21 pS at 50 mV up to 53 pS at V(m) = 200 mV, 20 degrees C. The concentration dependence of G(V(m)) corresponds to a Michaelis-Menten type saturat...

In this paper we describe transformation of Clavibacter michiganensis subsp. sepedonicus, the potato ring rot bacterium, with plasmid vectors. Three of the plasmids used, pDM100, pDM302, and pDM306, contain the origin of replication from pCM1, a native plasmid of C. michiganensis subsp. michiganensis. We constructed two new cloning vectors, pHN205 and pHN216, by using the origin of replication ...

Bacterial canker of tomato is the most damaging one and responsible for causing 70% yield losses each year. Green synthesis nanoparticles has ushered in a new research field known as green nanotechnology emerged vital tool management plant diseases. In comparison to other conventional techniques, various was found be eco-friendly, low-cost profitable resulting more stable synthesized materials....