Single-step and nested polymerase chain reactions (PCR) were used to determine the presence of Candidatus Liberibacter asiaticus, the phloem-limited bacterial pathogen of Huanglongbing (HLB), in leaves of wampee [Clausena lansium (Lour.) Skeels], lemon [Citrus limon (L.) Burm.] and several other citrus species. Specific PCR products were obtained when single-step PCR and nested PCR were used to...

Antibacterial and antifungal properties of wax and hexane extracts of Citrus spp. peels were tested using bioautographic and microdilution techniques against three plant pathogenic fungi (Penicillium digitatum, Curvularia sp., and Colletotrichum sp.), two human pathogens (Trichophyton mentagrophytes and Microsporum canis), and two opportunistic bacteria (Escherichia coli and Staphylococcus aure...

Mie-Gans (MG) fitting model theoretical utilizing the phenomena of scattering light to determine morphologies, shape, and size metallic nanoparticles in solution. In present work, average radius biosynthesized silver (AgNPs) was evaluated based on their Ultraviolet-visible (UV-Vis) spectra by MG for spherical non-spherical particles. Biosynthesis AgNPs using Lemon (Citrus Limon) leaves extract ...

Throughout the history of citrus production in Florida one of the diseases with a major impact has been the Asian form of citrus greening (HLB), which was first detected in 2005. As a response to this introduction, new survey and detection methods had to be developed utilizing field observations directed not only to corroborate symptoms already reported in the literature, but to describe any va...

Citrus produced in the southwestern United States is often irrigated with perchlorate-contaminated water. This irrigation water includes Colorado River water which is contaminated with perchlorate from a manufacturing plant previously located near the Las Vegas Wash, and ground water from wells in Riverside and San Bernardino counties of California which are affected by a perchlorate plume asso...

Unreduced (2n) gametes have played a pivotal role in polyploid plant evolution and are useful for sexual polyploid breeding in various species, particularly for developing new seedless citrus varieties. The underlying mechanisms of 2n gamete formation were recently revealed for Citrus reticulata but remain poorly understood for other citrus species, including lemon (C. limon [L.] Burm. f.). Her...

Piedra, is an asymptomatic fungal infection of the hair shaft, resulting in the formation of nodules of different hardness on the infected hair. The infection also known as Trichomycosis nodularis is a superficial fungal infection arising from the pathogen being restricted to the stratum corneum with little or no tissue reaction. The nodules are a concretion of hyphae and fruiting bodies of the...

Online quality assessment of various horticultural products using machine vision provides not only quick but also objective, consistent and quantitative measurement. Horticultural products of different sizes and shapes (circular or elliptical) are classified based on the area occupied, which is calculated by known geometrical method. Another factor in the classification is the detection of defe...