The possibility of using glycerol and glycerol-containing waste from biodiesel manufacture as a carbon and energy source for microbiological production of citric acid has been studied. Acid formation on the selective media had previously been tested in 66 yeast strains of different genera (Candida, Pichia, Saccharomyces, Torulopsis and Yarrowia). Under growth limitation by nitrogen, 41 strains ...

Citric acid and erythritol biosynthesis from pure and crude glycerol by three acetate-negative mutants of Yarrowia lipolytica yeast was investigated in batch cultures in a wide pH range (3.0-6.5). Citric acid biosynthesis was the most effective at pH 5.0-5.5 in the case of Wratislavia 1.31 and Wratislavia AWG7. With a decreasing pH value, the direction of biosynthesis changed into erythritol sy...

UNLABELLED BACKGROUND Although the mechanism of fentanyl-induced cough is unclear, several lines of evidence suggest that allergic mediators, such as histamine, may play a role in the production of fentanyl-induced coughs. The aim of this study was to explore the effects of fentanyl on cough sensitivity to inhaled citric acid and on histamine release in BALF in mice. METHODS The cough refl...

For evaluation of citric acid and citrate effects on bovine epididymal protoplasmic droplets, fifty bovine testes were collected in the October 2007 till June 2008 from Urmia slaughterhouse and transported to the laboratory in a cool container filled with 5 °C ice pack. Caudal epididymis was incised and sperm cells were put into Petri dishes containing hams f10 media with 10% fetal calf serum (...

The effect of nisin or citric acid or combinations of these two inhibitors on the inactivation of a cocktail of three Listeria innocua strains was investigated in a model brain heart infusion (BHI) broth and hummus (chickpea dip). In BHI broth, citric acid had a limited ability to inhibit L. innocua growth. Nisin initially reduced L. innocua concentrations by about 3 log cycles; however, L. inn...

We have shown that crude, cell-free extracts of Aspergillus terreus contain aconitase and cis-aconitic decarboxylase, and therefore quantitatively decarboxylate cis-aconitic, citric, and d-isocitric acids to itaconic acid and CO2 (1, 2). With use of labeled substrates, it has been shown that the isotope distributions in itaconic acid are consistent with the formation of cis-aconitic acid from c...

The objective of this experiment was to evaluate the effect of citric acid (CA), phytase and calcium in diets of laying hens on the productive performances and digestibility and excretion of phosphorus (P), calcium and nitrogen (N). An experiment was designed using 24 week old laying hens, which were fed a diet with a base of sorghum and soybean meal containing 2,700 kcal ME/kg, 15% crude prote...

OBJECTIVE The aim of this study was to evaluate the effect of citric acid irrigation on root fracture in different concentrations and at various time exposures on root fracture. MATERIALS AND METHODS Forty-eight human mandibular incisors with similar dimensions were selected. The specimens were decoronated, then divided into 6 groups as follows: A group without instrumentation and filling (G1...

The objective of this research was to evaluate the effects of immersion marination with citric acid, calcium chloride and combined mixture of citric acid and calcium chloride solutions on Semimembranosus meat quality traits. Meat cuts obtained from culled cows were marinated with 0.05 M citric acid, 0.2 M calcium chloride or the combined mixture of these two compounds for 24 hr. Nonmarinated me...

The specific adsorption of citric acid ions at hydroxyapatite interface was investigated by the means of radioisotope method (14C) as a function of citric acid ions concentration, NaCl concentration and pH. Application of the hydroxyapatite has become wide in the biomaterial field as the Ca10(OH)2(PO4)6 possess biocompatibility with human hard tissue. Hydroxyapatite was synthesized using three ...