The main objective of the present study was to develop an efficient and reliable probe to be routinely used for detection of chromosome 13 copy numbers by interphase FISH. To achieve this, a Yeast Artificial Chromosome (YAC) containing sequences specific for human 13q12 (744D11), was cultured and the whole yeast genomic DNA was extracted. The human insert within the isolated DNA was amplified b...

The chromosomal spread and karyotype of Bleak (Alburnus alburnus) from Anzali lagoon were identified using tissue squashing techniques with injection of 0.5ml/100g body weight of 0.01% Colchicines solution in fish fingerlings. Kidney and gill tissues were then extracted and chopped in KCl 0.045M for 20 min and fixed in Carnoy solution in 3 stages. The chromosomal spreads were stained in 20% Gim...

Eleven populations of five Avena species were analysed for meiotic characters including chiasma frequency and distribution as well as chromosomal association and segregation. Plants of a single population of A. eriantha showed the presence of 2n = 14 (diploid) and 2n = 4x = 28 chromosome number. Populations of A. barbata and A. wiestii possessed n = 14, while populations of A. sterilis ssp. lud...

 Abstract Background: To produce a reliable probe suitable for aneuploidy detection of chromosome 13 on uncultured lymphocytes and amniocytes by fluorescence in situ hybridization (FISH), we used a contig of three overlapping cosmids mapped to 13q12.3. Methods: The cosmid DNA carrying the expected sequences of human chromosome 13 was isolated from host cells and labelled with biotin-11-dUTP. Th...

The meiotic chromosome counts are reported in 10 species of the genus Cousinia including: C. adenostegia, C. antonowii, C. chaetocephala, C. dasylepis, C. eriobasis, C. freynii, C. prasina, C. rechingerorum, C. termei and C. trachyphyllaria from Iran. Specimens have been collected from different parts of Iran out of which, eight species are considered to be new and two provide confirmation of t...

background: high-energy ionizing radiation is harmful and changes the genetic makeup of dna, which can lead to increased risk of cancer. thus, the exposure of radiation dose should be under control and limited. ionizing radiation might lead to some chromosome aberrations like dicentric. there is a strong relation between the frequency of dicentric chromosome in metaphase, and the received dose....