Liquid chromatography (LC) may be simply described as an analytical technique used to separate the individual components of a solution based on their relative afRnities for a liquid mobile phase and a stationary phase. There are numerous modes of LC (i.e. reversed-phase, normal-phase, ion exchange) that are commonly classiRed according to the mechanism by which separation occurs. One such mecha...

In this article, selected examples of applications of liquid chromatography coupled to mass spectrometry are given. The examples include the analysis of i) impurities in manufactured, pharmaceutical or synthesis products, ii) polyphenols in natural products, and iii) phytohormones in plant extracts. Finally, examples of applications of molecular characterization via flow injection analysis by e...

An isocratic reversed-phase high-pressure liquid chromatographic method for the determination of six components of the teicoplanin complex in biological fluid was developed. By using fluorescence detection after precolumn derivatization with fluorescamine, the assay is specific and highly sensitive, with reproducibility studies yielding coefficients of variation ranging from 1.5 to 8.5% (at 5 t...