Proteoglycan monomers from the articular cartilages of the knee, hip and shoulder of 3 subjects (21, 26 and 45 years old) were isolated and analysed. The proteoglycan monomers from the high weight-bearing knee and hip joints were smaller than those from the low weight-bearing shoulder joints and both had a lower chondroitin sulphate content. The proteoglycan monomers from knee joint cartilage h...

ABSTRACT: Glycosaminoglycans (GAGs) are long-chain polysaccharides that divided into sulphates and non-sulphates, these being chondroitin sulphate, heparan dermatan heparin sulphate the only non-sulphate in group is hyaluronic acid. GAGs obtained from animal tissue by an expensive low-yield extraction process; however, they highly commercially valued exploited biomedical market. Their disacchar...

Human glucuronate 2-sulphatase (GAS), which is involved in the degradation of the glycosaminoglycans heparan sulphate and chondroitin 6-sulphate, was purified almost 2000000-fold to homogeneity in 8 0 yield from liver with a four-step six-column procedure, which consists of a concanavalin A-Sepharose/Blue A-agarose coupled step, a DEAE-Sephacel/octyl-Sepharose coupled step, CM-Sepharose chromat...

A histochemical study of sulphated glycosaminoglycans associated with the somites of the chick embryo SUMMARY A histochemical analysis has been made of sulphated glycosaminoglycans (mucopoly-saccharides) associated with chick embryo somites before and after the onset of overt cartilage differentiation. The sulphated glycosaminoglycans were distinguished and resolved into different types by the ...

Ordered conformations of proteoglycan-hyaluronic acid aggregates in the intercellular matrix in cartilage were observed by X-ray diffraction. The sodium salt form of three samples, (a) aggregated proteoglycan, (b) disaggregated proteoglycan and (c) reconstituted disaggregated proteoglycan, give essentially similar X-ray fibre-type diffraction photographs. The patterns correlate with the chondro...

BACKGROUND It is well documented that exercise reduces the risk of thromboembolic disease, possibly by increasing the plasma concentration of anticoagulant-antithrombotic compounds. OBJECTIVES As plasma glycosaminoglycans (GAGs) play a role in the anticoagulant-antithrombotic potential of plasma, to examine the concentration and profile of these compounds in well trained, long distance runner...

The aim of the present study was to develop a single unit, site-specific matrix tablets of aceclofenac allowing targeted drug release in the colon with a microbially degradable polymeric carrier, chondroitin suphate (CS) and to coat the optimized batches with a pH dependent polymeric. The tablets were prepared by wet granulation method using starch mucilage as a binding agent and HPMC K-100 as ...

Most biological molecules exhibit more than one function. In particular, many molecules have the ability to directly/indirectly scavenge free radicals and thus act in living organisms as antioxidant. During oxidative stress, the increase of these molecules levels seems to be a biological response that in synergism with the other antioxidant defence systems may protect cells from oxidation. Amon...

Chrondroitin sulphate proteoglycans obtained from embryonic chick bone marrow and epiphyseal cartilage were compared using immunochemical and biochemical analyses. Proteoglycans from each tissue, separated on CsCl density gradients, under dissociative conditions, into high (1.6 g ml-1), medium (1.5 g ml-1) and low (1.4 g ml-1) buoyant density fractions, were immunochemically analysed, using a p...

Cloned immortalized MC615 mouse chondrocytic cells were used to examine their capability to produce multiple types of matrix proteoglycans. Immunofluorescence staining indicated a uniform expression of aggrecan, biglycan and decorin by all cells. After culture with [35S]sulphate, proteo[35S]glycans secreted by the cells were found to elute in two peaks from a Sepharose CL-4B column. The first p...