With interest we read the article of Saito et al. that describes a severe case of chlamydiosis in humans (3). However, according to us two major points need to be clarified. The taxonomy of chlamydiae has been illustrated in 1999 by Everett et al. (1), and such classification is accepted universally, as evidenced by a number of manuscripts published in recent years. Classification is based on d...

With interest we read the article of Saito et al. that describes a severe case of chlamydiosis in humans (3). However, according to us two major points need to be clarified. The taxonomy of chlamydiae has been illustrated in 1999 by Everett et al. (1), and such classification is accepted universally, as evidenced by a number of manuscripts published in recent years. Classification is based on d...

The role of polymorphonuclear neutrophils (PMNs) in the development of the specific immune response against Chlamydophila abortus (Chlamydia psittaci serotype 1) infection was studied in a pregnant mouse model involving treatment with RB6-8C5 monoclonal antibody. PMN depletion significantly affected the immune response in the liver, in which the T-lymphocyte and F4/80(+) cell populations decrea...

Sera from free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) in the Indian River Lagoon, Florida (IRL) (n = 122), and the estuarine waters near Charleston, South Carolina (CHS) (n = 82) were collected from 2003 to 2007 and analyzed for antibodies to several bacterial and viral pathogens. Serological evidence of exposure to Chlamydophila psittaci; Eastern, Western, and Venezuelan equ...

A series of overlapping recombinant antigens, 61 to 74 residues in length, representing polymorphic outer membrane protein 90 (POMP90) of Chlamydophila abortus and two recombinant peptides spanning gene fragment p91Bf99 of POMP91B were assessed by immunoblotting to determine the antigen-binding sites of 20 monoclonal antibodies to POMP90, -91A, and -91B. The epitopes were further restricted by ...

Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%, whereas, in case of rams seroprevalence was 0% by using complement fixation test. Using iELISA in the same groups the seroprevalences observed were, 24.24% in goats, 11.30% in sheep and 5.30% in r...

Ovine chlamydiosis, also known as enzootic abortion of ewes (EAE) or ovine enzootic abortion (OEA), is caused by the bacterium Chlamydophila abortus. Chlamydial abortion typically occurs in the last 2–3 weeks of pregnancy with the appearance of stillborn lambs and inflamed placentas. However, infection can also result in the delivery of full-term stillborn lambs or weak lambs that do not surviv...

Chlamydia trachomatis is the leading cause of preventable blindness and bacterial sexually transmitted diseases worldwide. Plaque assays have been used to clonally segregate laboratory-adapted C. trachomatis strains from mixed infections, but no assays have been reported to segregate clones from recent clinical samples. We developed a novel shotgun cell culture harvest assay for this purpose be...