A rapid and sensitive dot-ELISA test was developed for the simultaneous detection of antibodies against infectious bursal disease (IBD), hydropericardium syndrome (HPS) and chicken anaemia (CAV) viruses in a single serum sample. The developed dot-ELISA was compared with the virus neutralization test (VNT). For IBD and HPS, viruses isolated and purified from field outbreak materials were used wh...

*Corresponding author: Dr. Marinko Vilić, DVM Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, HR-10000 Zagreb, Croatia Fax: +385 12390174 E-mail: [email protected] Background: The specific antibody response against Newcastle disease virus (NDV) in the blood serum of chickens hatched from eggs exposed to low dose gamma-radiation ...

Chicken serum promotes migration of cardiac mesenchymal cells of chick embryos in vitro. In the present study, migration promotion of unknown migration promoters in chicken serum was examined by using lectins. Cardiac mesenchymal cells of the conotruncal and atrioventricular cushions were cultured on collagen type-I gel with medium including chicken serum. A concentration (100 micrograms/ml) of...

This study was conducted to evaluate the effect of feed restriction on some biochemical parameters-related to growth in white Pekin duck. Therefore, fifty white Pekin ducks were allocated into two equal groups as ad libitum and feed restriction group, in which each one was arranged in five replicates. The birds were allowed to free feed access from 1 to 7 days and from 15 to 49 days of age and ...

Aim: This study was aimed to develop the disease resistance broiler chicken lines over two generations (G , G and G ) of 0 1 2 selection for immunocompetence (IC) index by targeting all the facets of immune response traits viz., humoral response (HR) to Sheep red blood cells (SRBC), cell mediated immune response (CMI) to phytohaeagglutinationP mitogen (PHA-P), levels of serum serum immunoglobin...

Chicken soup has long been regarded as a remedy for symptomatic upper respiratory tract infections. As it is likely that the clinical similarity of the diverse infectious processes that can result in "colds" is due to a shared inflammatory response, an effect of chicken soup in mitigating inflammation could account for its attested benefits. To evaluate this, a traditional chicken soup was test...

In order to determine the involvement of nitric oxide in the pathogenesis of coccidiosis induced by Eimeria, 30 chickens were challenged with mixed sporulated oocysts of four species of Eimeria (E. acervulina, E. maxima, E. necatrix, and E. tenella) at 26 days of age. There was an increasing of oocyst shedding in the infected birds at 6, 10 and 14 days post-infection. Histopathological examinat...

Survival of the chick depends upon the transfer of maternal IgG from the egg yolk into the fetal circulation. Radiolabeled chicken IgG binds specifically to both fixed and unfixed yolk sac tissue from day 7 or 8 of embryogenesis through at least day 18. This binding is saturable, Fc-specific, pH-dependent, and reversible. Chicken Fc fragments and some heterologous IgGs compete with IgG binding,...

Isolated porcine pepsinogen A was used for the preparation of polyclonal rabbit and polyclonal chicken anti-pepsinogen A antibodies. Immunochemical properties of both immunoglobulin fractions were compared. The rabbit anti-serum was further purified using immobilized porcine pepsinogen A on magnetic cellulose beads and the resulting anti-pepsinogen A fraction proved to be applicable for the sep...