Amniotic fluid at 15-20 weeks contains high concentrations of microvillar enzymes which serve as markers for the prenatal diagnosis of cystic fibrosis. We found that these enzymes were mostly bound to amniotic fluid particulate fraction which eluted in the void volume on Sepharose 6B chromatography and precipitated in ultracentrifugation at 100,000g. Electron microscopy showed that the particul...

During the second and third trimesters increasing activities of the glycolytic enzyme pyruvate kinase are to be found in human amniotic fluid. At term (37 to 42 weeks), a very steep rise in amniotic fluid pyruvate kinase can be noticed, which may serve as an additional prenatal indicator of fetal maturity. The presumable main sources of the enzyme in amniotic fluid are the epithelium cells of t...

BACKGROUND Myocardial dysfunction occurs in a variety of fetal disorders. Findings from adult cardiology, where n-terminal pro-B-type natriuretic peptide (nt-proBNP) is an established biomarker of left ventricular dysfunction have been extended to fetal life. Since fetal blood sampling is technically challenging we investigated amniotic fluid nt-proBNP for its suitability to diagnose fetal myoc...

Aim. The aim of this study is to investigate whether amniotic fluid leptin and insulin levels can be used for prediction of for prediction of gestational diabetes mellitus or not. Methods. The levels of leptin and insulin were measured in the samples of amniotic fluid which were obtained at 16-20 weeks of pregnancy during genetic amniocentesis. We recorded the patients who developed gestational...

OGAWA, Y. Fetal Pulmonary Surfactant in Amniotic Fluid of Ewes. Tohoku J. exp. Med., 1972, 108 (4), 307-315-The phospholipids in the amniotic fluid from healthy pregnant ewes at various stages of gestation were analyzed in relation to the fetal pulmonary surfactant. The most abundant phospholipid was phosphatidylcholine which increased as the term approached. Analysis on the positional specific...

The purpose of this investigation was to study and compare the electrophoretic patterns of optic lensproteins of different species of domestic animals at pre- and post-natal ages. Optic lenses were removed from the embryo or adult sheep, cattle, goat, camel and chicken at the slaughter-house then homogenized and subjected to sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). I...

background: adhesion is a common complication of gynecology surgery so different barrier agents and solutions have been used during these operations to separate and protect tissues from adhesion after surgery. adept is one of these solutions that have been postulated to reduce the chance of adhesion following gynecolgy surgery. objective: to evaluate the effect of 4% icodextrin in reducing adhe...

OBJECTIVE To describe the association between echogenic amniotic fluid and first-trimester fetal acrania. METHODS Nine fetuses with acrania were examined between 11 weeks' and 13 weeks 6 days' menstrual age for the presence of echogenic free-floating particles in the amniotic fluid. Cases were classified into 3 types according to the echogenicity of the amniotic fluid: similar to (type 0), sl...

Human fibronectin was isolated from second-trimester amniotic fluid, from amniotic fluid obtained at term and from adult plasma. The amniotic-fluid fibronectins had a slightly higher apparent molecular weight on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis than the plasma fibronectin. Early- and late-amniotic-fluid fibronectin had 9.5 and 9.6% carbohydrate respectively, whereas pl...

Ingestion of amniotic fluid and placenta by rats has been shown to enhance opioid-mediated antinociception but not affect the nonopioid-mediated antinociception produced by aspirin, suggesting specificity for opioid-mediated processes. However, enhancement by the active substance(s) in amniotic fluid and placenta (POEF, for placental opioid-enhancing factor) of antinociception produced by other...