نتایج جستجو برای: chemostat culture

تعداد نتایج: 278509  

2013
Celeste J. Brown Jack Millstein Christopher J. Williams Holly A. Wichman

Observing organisms that evolve in response to strong selection over very short time scales allows the determination of the molecular mechanisms underlying adaptation. Although dissecting these molecular mechanisms is expensive and time-consuming, general patterns can be detected from repeated experiments, illuminating the biological processes involved in evolutionary adaptation. The bacterioph...

Journal: :Journal of hazardous materials 2007
Kuo-Ching Lin Yu-Long Lee Chung-Yuan Chen

This study presents the metal toxicity as revealed by Chlorella pyrenoidosa using a short-term continuous test and the conventional batch test. Toxicity tests were conducted in a chemostat with a test duration of 1 day. Toxicity data of five different metals (Cd, Pb, Co, Ni, and Zn) derived from the continuous test were compared against results from the conventional batch tests. The batch test ...

2014
Mauricio Vergara Silvana Becerra Julio Berrios Nelson Osses Juan Reyes María Rodríguez-Moyá Ramon Gonzalez Claudia Altamirano

Mild hypothermia condition in mammalian cell culture technology has been one of the main focuses of research for the development of breeding strategies to maximize productivity of these production systems. Despite the large number of studies that show positive effects of mild hypothermia on specific productivity of r-proteins, no experimental approach has addressed the indirect effect of lower ...

2015
Mauricio Vergara Julio Berrios Irene Martínez Alvaro Díaz-Barrera Cristian Acevedo Juan G. Reyes Ramon Gonzalez Claudia Altamirano Salvatore V Pizzo

BACKGROUND Chinese hamster ovary (CHO) cells are the main host for producing recombinant proteins with human therapeutic applications mainly because of their capability to perform proper folding and glycosylation processes. In addition, mild hypothermia is one of the main strategies for maximising the productivity of these systems. However, little information is available on the effect of cultu...

2014
Hendrik Gideon Brink Willie Nicol

BACKGROUND Succinic acid is well established as bio-based platform chemical with production quantities expecting to increase exponentially within the next decade. Actinobacillus succinogenes is by far the most studied wild organism for producing succinic acid and is known for high yield and titre during production on various sugars in batch culture. At low shear conditions continuous fermentati...

2003

Sporulation of Bacillus subtilis 168 was studied in chemostat cultures. Sporulation occurred at high frequency under limitation of growth by glucose or the nitrogen source in minimal medium, whereas rates of sporulation were low for Mg'+, phosphate, citrate, or tryptophan limitation. Sporulation was found at all growth rates tested, and the incidence of spores increased with decrease in growth ...

Journal: :Journal of applied microbiology 2000
D E Norwood A Gilmour

A constant-depth film fermenter (CDFF) was used to culture a steady-state multispecies biofilm consisting of one strain each of Listeria monocytogenes, Pseudomonas fragi and Staphylococcus xylosus. These bacteria were initially grown together in a conventional chemostat to achieve a steady state before being inoculated into the CDFF over an 18-h period. A dilute tryptone soya broth (TSB) medium...

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