a sensitive and fast chemiluminescence (cl) method has been described for the direct determination of atropine. the method is based on the quenching effect of atropine on the cl signals of the luminol– h2o2 reaction catalyzed by hemin in an alkaline medium. hemin could strongly enhance the chemiluminescence of the luminol–h2o2 system. the luminophor of the luminol–h2o2–hemin cl system was ident...

A rapid and sensitive chemiluminescence microfluidic immunoassay system based on super-paramagnetic microbeads for determination of alpha-fetoprotein (AFP) is described. In this system we use CO(2) laser to fabricate microfluidic chip, and use super-paramagnetic microbeads as solid carrier of antibody, chemiluminescence as detection signal. With this system we can perform AFP analysis within 20...

An ultrasensitive and rapid sandwich-type chemiluminescence immunoassay (CLIA) was developed for the clinical determination of human epididymis protein 4 (HE4) in human serum, using GoldMag nanoparticles as solid phase and acridinium ester (AE) as chemiluminescence system (GMP-CLIA). The process of AE labeling antibodies was systematically studied and evaluated. The effect of varies factors suc...

a chemiluminescence (cl) method has been developed for the determination of hydroxyzine (hyd) and its metabolite cetirizine (cet). the method is based on sensitizing effect of cet and hyd on the weak cl reaction between ru(phen)32+ and acidic ce(iv). a mechanism for the cl reaction has been proposed on the basis of uv-vis, fluorescent and cl spectra. by using the recommended procedure, the cali...

Reaction mechanisms of various kinds of photocatalysts have been reviewed based on the recent reports, in which various spectroscopic techniques including luminol chemiluminescence photometry, fluorescence probe method, electron spin resonance (ESR), and nuclear magnetic resonance (NMR) spectroscopy were applied. The reaction mechanisms elucidated for bare and modified TiO2 were described indiv...

Molecular detection of HBV has a significant impact on prognosis and therapy of the disease. In this paper, a sensitive nucleic acid detection method of HBV was established taking advantage of magnetic nanoparticles (MNPs), chemiluminescence (CL) and polymerase chain reaction (PCR). HBV-DNA was extracted from hepatitis B positive human blood samples using MNPs adsorption method and biotin was l...