The purification of heparin from offal is an old industrial process for which commercial recipes date back to 1922. Although chemical, chemoenzymatic, and biotechnological alternatives for this production method have been published in the academic literature, animal-tissue is still the sole source for commercial heparin production in industry. Heparin purification methods are closely guarded in...

The polypeptide corresponding to the amino acid sequence from residue 6 through 47 in staphylococcal nuclease has been synthesized by the solid-phase method. The synthetic product closely resembles the corresponding native polypeptide in both physical and chemical properties. The synthetic peptide may be recombined with the complimentary native peptide comprising residues 49 through 149 to form...

We have partially purified human estrogen receptor from MCF-7 breast cancer cells in order to further characterize the chemical and biological properties of the receptor and to prepare specific receptor antibodies for radioimmunoassay. The estrogen receptor from MCF-7 cell cytosol was purified 1,166-fold (27% recovery) over starting cytosol by combining ammonium sulfate precipitation, affinity ...

In the field of physical sciences and in chemical process technology, the purification of materials is very often most important. In many situations conventional processes such as distillation and crystallization are not applicable as in the case of closely boiling liquids, organic dyes, biologicals and colloids. I n such cases, the less conventional techniques assume a great importance. Of the...

The purification of endohedral metallofullerenes by high performance liquid chromatography is very time-consuming and expensive. A number of rapid and inexpensive non-chromatographic methods have thus been developed for large-scale purification of metallofullerenes. In this review, we summarize recent advances in non-chromatographic purification methods of metallofullerenes. Lewis acid-based co...

The treatment of spent oil-in-water emulsion (SOWE) from a non-ferrous metal-processing plant by using aluminum sulfate and hydrated lime was studied in order to determine the purification efficiency, optimize the operating conditions and scale up the treatment process. The purification efficiency was estimated by comparing the compositions of the SOWE and the processed wastewater. The treatmen...

Self-decontaminating materials are defined as substances or objects designed and fabricated to deactivate pathogenic microorganisms or chemical warfare agents. Self-decontamination makes it possible for continuous protection against toxic materials. In order to develop destructive barriers against biological and chemical air pollutants, protective clothing and membranes using catalytic or enzym...

bacillus thuringiensis (bt) has been extensively used in biopesticidal formulations due to its safe environmental and human health records. the widespread use of bt is often challenged by production as well as formulation costs which are in direct contact with downstream processing, i.e. the separation/purification step. downstream separation/purification efficacy governs the marketability of a...