In collaboration with the Armed Forces Institute of Pathology's Department of Defense DNA Registry, the National Institute of Standards and Technology recently evaluated the performance of a short tandem repeat multiplex with dried whole blood stains on four different commercially available identification card matrixes. DNA from 70 stains that had been stored for 19 months at ambient temperatur...

Toxoplasmosis, caused by Toxoplasma gondii, is an important parasitic disease worldwide. Different techniques have been developed for T. gondii detection. At present, polymerase chain reaction (PCR) has been widely used. However, PCR for identifying T. gondii remains unsatisfactory in many laboratories because of lack of standardization and variations in efficiency. In the present study, we opt...

*Correspondence: Richar Rodriguez-Hidalgo, Central University of Ecuador, Faculty of Veterinary Medicine, International Centre for Zoonoses, Av. America s/n., 170517 Quito, Ecuador e-mail: [email protected] Modifications to the DNA isolation protocol from Cochliomyia spp., are suggested based on the Chelex® 100 reactive. To apply the sterile insect technique (SIT) program it is necessary to...

Artemia is one of the most conspicuous invertebrates associated with aquaculture. It can be considered as a model organism, offering numerous advantages for comprehensive and multidisciplinary studies using morphologic or molecular methods. Since DNA extraction is an important step of any molecular experiment, a new and a rapid method of DNA extraction from adult Artemia was described in this s...

Rat liver mitochondria were found to hydroxylate E-Ntrimethyl+lysine to produce P-hydroxy-c-N-trimethyl-L-lysine, an intermediate in carnitine biosynthesis. The hydroxylating system requires a-ketoglutarate, Fez+, and ascorbate, but does not require NADPH nor NADH. No activity was found in the microsomal or soluble fractions of liver extracts. The hydroxylated a-amino acid was isolated and char...

The most essential limitation of batch-injection analysis (BIA) methodology compared to other flow methods (CFA, FIA, SIA) is the lack of possibility of on-line sample processing in the measuring system. Some procedures of on-line sample pretreatment in BIA are possible by changing the plastic tip of the automatic micropipette used for sample injection into a flow-through reactor, e.g. by packi...

Nucleic acid amplification technologies offer great promise for the rapid, sensitive and specific diagnosis of tuberculosis. However, the isolation of inhibitor-free DNA from biological specimens is a bottleneck of the PCR assay. Here we describe a simple method for the isolation of PCR-amplifiable DNA of Mycobacterium tuberculosis from all types of samples of pulmonary and extrapulmonary origi...

A method integrating a long waveguide capillary cell with a preconcentration resin in a multi-syringe flow injection analysis (MSFIA) system for iron determination in waters was developed. The determination of iron is based on a colorimetric reaction and two reagents were tested, ferrozine and ammonium thiocyanate. A liquid waveguide capillary cell (1.0 m pathlength, 550 μm i.d. and 250 μL inte...

dna extraction with an appropriate quality and quantity is the first step in molecular identification of some group of insects that their identification morphologically is difficult and dependent on a particular life stage, size or gender. selection of a suitable protocol is a key factor to provide a pure dna. in this study quality and quantity of five extraction methods used in entomological l...