A rapid method for the determination of chondroitin sulfate in raw materials was developed. The samples were finely powdered, dissolved in water, and injected directly into the liquid chromatograph. The method used a C18 column, a wavelength of 195 nm, and a mobile phase containing octane sulfonic acid. The method gave results that were slightly different from those generated by atitrimetric me...

(1) Polysaccharides were isolated from bovine liver capsule by extraction with 2m-potassium chloride followed by precipitation from 0.8m-potassium chloride with cetylpyridinium chloride. Chondroitin sulphate was eliminated by digestion with hyaluronidase. The yield of heparin was approx. 40% of that obtained after extraction of the papain-digested tissue. (2) The macromolecular properties of th...

ORII, T., YAMAGUCHI, M., MINAMI, R. and NAKAO, T. Decrease of Urinary Acid Mueopolysaccharides in Hurler's Syndrome after Plasma Infusion. Tohoku J. exp. Med., 1974, 112 (4), 385-387 The fresh plasma infusion in patients affected by Hurler, Scheie or Morquio syndromes was followed by a decreased urinary excretion of chondroitin sulfate B or keratosulfate. Furthermore, in all of the patients the...

1. A procedure for the isolation and purification of the heparin-protein complex from ox liver capsule, based on the solubility properties of mucopolysaccharide-cetylpyridinium complexes, is described. 2. The yield of the heparin-protein complex with this method averages 35mg./100g. of dry ox liver capsule. 3. The results of analyses on the polysaccharide show good agreement with values previou...

This article demonstrates, for the first time, construction of novel cucurbituril (CB)-adorned supramolecular micellar assemblies of a cationic surfactant, cetylpyridinium chloride (CPC), through noncovalent host-guest interactions. The distinct cation receptor features and cavity dimensions of the CB5 and CB7 homologues assert that the macrocyclic hosts remain complexed with the CPC monomers a...

Micellar complexes were prepared from bacteriochlorophyll a and bacteriopheophytin a with the cationic detergents, cetyltrimethyl ammonium bromide and cetylpyridinium chloride. These complexes have spectroscopic properties (absorption, circular dichroism) which are very different from the ones formed with non-ionic detergents like Triton X-100, and also with anionic detergents. Bacteriochloroph...

The interaction of phospholipid membranes and oligonucleotides complexed with a positively charged surfactant is reported. Phospholipid membranes were assembled at the interface between an immobilized organic phase and an aqueous phase using the Langmuir-Blodgett (L-B) technique. The interaction and adsorption of the naked oligonucleotides and oligonucleotides complexed with cetylpyridinium chl...