The purpose of this study was to determine whether glycylsarcosine (a model dipeptide) and oseltamivir (an antiviral prodrug) exhibited a species-dependent uptake in yeast Pichia pastoris expressing the rat, mouse, and human homologs of PEPT1. Experiments were performed with [(3)H]glycylsarcosine (GlySar) in yeast P. pastoris expressing human, mouse, and rat peptide transporter 1 (PEPT1), in wh...

The SNAP was modified to SNAP ST (Idexx Laboratories Inc., Westbrook, ME), a new 7-8 minute test which no longer requires the use of an incubator. This new test was validated at ILVO-T&V according to Commission Decision 2002/657/EC and the CRL Guidelines for the Validation of Screening Methods (Anon., 2010). The SNAP ST is very specific as an interference was only caused by clavulanic acid, a β...

These studies extend the recent observation that cefazolin is inactivated to a greater extent than cephaloridine by some strains of penicillinase-producing Staphylococcus aureus, whereas cephalothin undergoes little if any inactivation. In Mueller-Hinton broth (inoculum, 3 x 10(6)) 100 recently isolated strains had minimal inhibitory concentrations (MICs) </= 2 mug/ml for cephalothin and cephal...

The purpose of the study was determining the in vitro sensitivity of Streptococcus agalactiae, other Streptococcus sp., Staphylococcus aureus, coagulase-negative staphylococci, Escherichia coli, and Arcanobacterium pyogenes strains to antibiotics commonly used in veterinary praxis. These pathogens were isolated from cows with clinical or subclinical mastitis in 2006–2007. The activity of 10–14 ...