objective: this study presents a simple method for isolation, expansion and purification of neonatal mouse spermatogonial stem cells. methods: we used enzymatic digestion to isolate a cell suspension of spermatogonia and sertoli cells from neonatal 2-day-old mice. the cells were cultured in dmem/f12 that contained 10% serum for two weeks. sertoli and spermatogonia cell characteristics were conf...

Several hydrolytic enzyme activities were detected in the wall of developing cells of Rubus fruticosus in suspension culture. The corresponding substrates of the enzymes are mostly polysaccharide wall constituents, except for chitinase activity. The activities measured when the enzymes were in the free state or wall-bound showed the positive influence of the cell wall micro-environment. Changes...

Cell-suspension cultures of Capsicum annuum L., Solanum aculeatissimum Jacq. and, Datura fastuosa L. (Solanaceae) were established and investigated for the ability to biotransform exogenously-supplied hydroquinone to arbutin. All cultures exhibited good 24-day growth in Murashige and Skoog medium, supplemented with 2,4-dichlorophenoxyacetic acid and benzylaminopurine as plant growth regulators,...

Transfection of suspension cells has proven to be very difficult using conventional methods. Here, we present a simple and time-saving new transfection protocol wherein cell culture plates coated with chicken egg white are seeded with suspension cells prior to transfection. Our results demonstrate that coupling egg white coatings with commercially available transfection reagents leads to high t...

zinc and iron nano-oxides (100 ppb) were promoted the hypericin and hyperforin production in hypericum perforatum cell suspension culture. high performance liquid chromatography method was used for detectection and identification of hypericin and hyperforin in h. perfuratum cell suspension cultures elicited with different concentrations of zinc and iron nano-oxide (0, 50, 100 and 150 ppb) after...

Cell surface antigens used as markers of in vivo differentiation may not be stable on monocytes maintained under different conditions of in vitro culture. Monocytes were isolated from blood by centrifugation over Percoll or by adherence to plastic dishes, and the cells cultured in suspension or as adherent monolayers. Initially, monocytes obtained by both methods were similar in size, morpholog...

background: specific growth factors and feeder layers seem to have important roles in in vitro embryonic stem cells (escs) differentiation. in this study,the effects of bone morphogenetic protein 4 (bmp4) and mouse embryonic fibroblasts (mefs) co-culture system on germ cell differentiation from mouse escs were studied. materials and methods: cell suspension was prepared from one-day-old embryoi...

In a tissue culture isolate from a biopsy of a recurrent paraganglioma, lymphoblast-like cells were found growing in suspension from the first culture on. The suspension culture has now been growing continuously for 14 months. These cells occasionally contained cytoplasmic areas of anastomosing tubular structures showing continuity with endoplasmic reticulum. Cell-to-cell contact between these ...