Senile plaques, a neuropathological feature of Alzheimer's disease, consist mainly of insoluble aggregates of pamyloid protein I I I . Using rat pheochromocytoma PC12 cells as an irr vitro model. i t has been shown that 8-amyloid potently inhibits the reduction of MTT 12.31. The cellular reduction of M 7 T is thought to be mediated by the mitochondrial respiratory chain, although other NAD(P)H ...

Nitric oxide (NO) plays a key role in many physiological and pathophysiological events, including the control of cell respiration. Both reversible and irreversible inhibition of mitochondrial respiration have been reported following the generation of NO by cells. We have exposed the murine macrophage cell line J774 to high concentrations of NO, such as are generated in some pathological conditi...

Many solid tumor cells exhibit mitochondrial respiratory impairment; however, the mechanisms of such impairment in cancer development remain unclear. Here, we demonstrate that SNU human hepatoma cells with declined mitochondrial respiratory activity showed decreased expression of mitochondrial 8-oxoguanine DNA glycosylase/lyase (mtOGG1), a mitochondrial DNA repair enzyme; similar results were o...

The article presents the results of an in vitro study synergetic effect evaluation combined preparation based on coordination complex ethylmethylhydroxypyridine succinate and trimethylhydrazinium propionate energy metabolism cell respiration. aim was to evaluate mitochondria-directed action metabolic antioxidant succinic acid with relation optimizing cells under oxidative stress conditions, as ...

Cell-based biosensors (CBBs) are becoming important tools for biosecurity applications and rapid diagnostics in food microbiology for their unique capability of detecting hazardous materials. Pollutants, such as heavy metals and chemicals, are now considered as a global threat and are associated with detrimental health outcomes. Fast and accurate detection of pollutants is essential to reduce t...

This Personal Account highlights the capabilities of spontaneous Raman microspectroscopy for studying fundamental biological processes in a single living cell. Raman microspectroscopy provides time- and space-resolved vibrational Raman spectra that contain detailed information on the structure and dynamics of biomolecules in a cell. By using yeast as a model system, we have made great progress ...

The development of a new anticancer drugs targeted at energy metabolism tumor cells is promising­ approach for cancer treatment. aim our study was to investigate the action thiazole derivative N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1-benzofuran-2-carboxamide (BF1) and its complex with PEG based polymeric nanoparticle (PEG-PN) on respiration mitochondrial membrane potential in murine NK/Ly c...

During aerobic respiration, microorganisms consume oxygen (O2) through the use of different types of terminal oxidases which have a wide range of affinities for O2. The Km values for O2 of these enzymes have been determined to be in the range of 3 to 200 nmol liter(-1). In this study, we examined the time course of development of aerobic respiratory kinetics of four marine bacterial species (Di...