Background and Aims: World Health Organization Global Polio Laboratory Network (GPLN) plays a critical role in the Global Polio Eradication Initiative. Cell culture methods (mostly RD and L20B cell lines) have been used for Enteroviruses and polioviruses isolation, respectively. Cross-contamination among L20B and RD cell lines causes the problem in accuracy of poliovirus surveillance and decrea...

apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development and tissue homeostasis. caspases, a family of cysteine-dependent aspartate-directed proteases, play a critical role in the initiation and execution of apoptosis. in this study, cytotoxicity and apoptogenic effect of 2-phenyl 4-carboxamide derivatives were evaluated in sknmc (huma...

the effect of natural killer (nk) cells and lymphokine activated killer ( lak) cells was studied on a group of human melanoma cell lines. peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhi l-2 for producing lak cells. the cytotoxicity of effector cells was quantified using a 4 hour si determining the density of antigen expression on tumor cel...

the sporozoites of theileria annulata invade bovine mhc ii cells, where they differentiate into schizonts. the later can immortalize and induce fundamental changes in their host cells. live attenuated vaccine is an important way of controlling t. annulata infection of cattle. production is by prolonged cultivation of macroschizont-infected cells. the mechanisms underlying this transformation ar...

background: numerous molecules in papaveraceae family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few cases. objective: numerous molecules in this family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few...

marine streptomyces are prolific producers of majority of bioactive secondary metabolites which are used in pharmaceutical industry as effective drugs against life threatening diseases. the cytotoxic activity of the pure compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester (dmehe) from marine derived actinomycete streptomyces sp. vitsjk8 was investigated against mouse embryonic fi...

In many model systems, cystic fibrosis (CF) phenotype airway epithelial cells in culture respond to P. aeruginosa with greater interleukin (IL)-8 and IL-6 secretion than matched controls. In order to test whether this excess inflammatory response results from the reported increased adherence of P. aeruginosa to the CF cells, we compared the inflammatory response of matched pairs of CF and non C...

in many acute leukemias, normal differentiation does not occur. however, in many cell lines derived from hematologic malignancies, differentiation or apoptosis can be induced by variety of agents. despite advances in the treatment of acute lymphoblastic leukemia (all), in most patients long-term survival rates remain unsatisfactory, especially in t-cell derived all. thus we studied the anti-can...

Weak cell-surface adhesion of cell lines to tissue culture surfaces is a common problem and presents technical limitations to the design of experiments. To overcome this problem, various surface coating protocols have been developed. However, a comparative and precise real-time measurement of their impact on cell behavior has not been conducted. The prostate cancer cell line LNCaP, derived from...