Epidemiologic studies have demonstrated that Helicobacter pylori infection is associated with increased risk for the development of gastric cancer. Animal studies have also shown that H. pylori infection leads to gastric carcinogenesis, especially intestinal phenotypes. However, no in vitro study has been carried out for cell transformation induced by H. pylori. The present study aimed to inves...

Objective(s): To explore the correlation between expression patterns and functions of miR-383 and LDHA in hepatocellular cancer (HCC). Materials and Methods: We detected the expression of miR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate produc...

Antioxidant compounds inhibit formation of free radicals, chelate catalytic metals, and scavenge free radicals in biological systems. In addition, antioxidants play a decisive role in prevention of numerous physiological dysfunctions, cancers, and metabolic disorders. This study sought to evaluate the antioxidant capacity and cytotoxic effect of grape seed extract (GSE), crocin (CRO), and pheny...

CCAAT enhancer binding protein alpha (CEBPA, also known as C/EBPα) is a transcription factor that plays an essential role in regulating terminal differentiation and cell proliferation of many tissues. The objective this study to explore the potential function CEBPA ovarian cancer. expression cancer samples adjacent normal tissues was evaluated by qRT-PCR. putative cells immunohistochemistry, we...

April M. Single strand breaks (SSBs) are among the most frequent DNA lesions caused by endogenous and exogenous agents. The most utilized alkaline-based assays for SSB detection frequently give false positive results due to artifactual SSBs arising from cleaved alkali-labile sites. Here we developed a specific SSB assay using alkaline gel electrophoresis (AGE) coupled with an O-hydroxylamine, O...

Purpose: Trophoblastic cancers (Choriocarcinoma, testicular and ovarian germ cell malignancies) secrete hyperglycosylated hCG and non-trophoblastic cancers secrete hyperglycosylated hCG free βsubunit.Both are structural variants of hCG independent to the hormone hCG. They are autocrines acting on a transforming growth factor β-II (TGF-β-II), receptor not acting on the hormone receptor. Here the...

Curcumin (CUR) is the active curcuminoid with many physiological, biochemical, and pharmacological properties. Solubility and stability of CUR is the limiting factors for realizing its therapeutic potential. Bovine β-casein is an abundant milk protein that is highly amphiphilic and self-assembles into stable micellar nanoparticles in aqueous solution. β-Casein nanoparticle can solubilize CUR mo...

In type 1 diabetes (T1D), β-cell loss is silent during disease progression. Methylation-sensitive quantitative real-time PCR (qPCR) of β-cell-derived DNA in the blood can serve as a biomarker of β-cell death in T1D. Amylin is highly expressed by β-cells in the islet. Here we examined whether demethylated circulating free amylin DNA (cfDNA) may serve as a biomarker of β-cell death in T1D. β cell...

In this study, the stem bark of Artocarpus kemando was used to find alternative antioxidants from natural sources with fewer side effects. A. wasextracted successively using hexane, chloroform and methanol solvents, evaluated for antioxidant, cytotoxic, antiproliferative activities. The extracts were investigated determination their total phenolic content (TPC) flavonoid (TFC). Then, antioxidan...