The Front Cover shows the bioconjugation of an antiproliferative prochelator to serum albumin via formation a disulfide bond with reduced cysteine on protein. abundance in blood is represented by red background featuring cells. disulfide-masked prochelators this class have activities at submicromolar levels panel cancer cell lines. design Matthew W. Lluis. More information can be found Communic...

Objective: The aim of this study is to investigate the neuroprotective effect chrysotoxine (CTX) on sevoflurane-treated nerve cells and uncover potential regulation mechanism. Methods: Nerve treated with sevoflurane CTX were subjected MTT apoptotic detection. Cell apoptosis oxidative stress detected by flow cytometry (FCM) ELISA assays. In addition, immunoblot assay was performed signaling path...

fast enzyme linked immunosorbent assay (fast-elisa) was compared with the standard elisa for the diagnosis of human hydatidosis. seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), tric...

the witches’ broom disease of lime trees is a destructing disease caused by the bacterium candidatus phytoplasma aurantofolia (1). the disease is prevalent in oman,united arab emirates and iran. to restrict the spread ofthe infected trees, detection of the causative bacteriumis crucial. besides pcr-based methods, antibodies can beapplied for detection of pathogen-infected cells.recently shahrya...

Background: Human CD34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (HSCs) and the small- vessel endothelial cells of a variety of tissues. CD34 plays a critical role as a marker for diagnosis and classification of leukemia. Anti CD34 antibodies are used for isolation and purification of HSCs from bone marrow, peripheral blood and cord blood. Objective: ...

background: human cd34 is a transmembrane glycoprotein which is expressed in human hematopoietic stem cells (hscs) and the small- vessel endothelial cells of a variety of tissues. cd34 plays a critical role as a marker for diagnosis and classification of leukemia. anti cd34 antibodies are used for isolation and purification of hscs from bone marrow, peripheral blood and cord blood. objective: t...

A rapid and reproducible enzyme-linked immunosorbent assay (ELISA) is described for determining antibodies in human sera against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The sera were absorbed for 30 min with heterologous virus-infected-cell extracts to remove cross-reacting antibodies and then were applied to ELISA plates containing the target antigens, immunoaffinity-purified H...

Citation: Boughattas S (2015) Commentary on: “Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran”. Front. Microbiol. 6:215. doi: 10.3389/fmicb.2015.00215 Commentary on: “Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat b...

African swine fever (ASF) is a notifiable highly infectious lethal haemorrhagic diseasein domestic pigs. Indirect enzyme-linked immunosorbent assays (ELISA). was carried out to detect antibodies against ASF virus and haematological investigations were also on blood samples collected from one hundred eighty seven pigs various piggries slaughter houses selected Local Government Areas in Benue Sta...