of Lectures 15 & 16: Library: A genomic library is a population of host bacteria, each of which carries a DNA molecule that was inserted into a cloning vector, such that the collection of cloned DNA molecules represents the entire genome of the source organism. The DNA molecules of an organism of interest are isolated, partially digested by an endonuclease restriction enzyme and ligated into ve...

A new retroviral system has been developed for the generation of a cDNA library and the functional cloning of tumor antigens. These retroviral vectors contain a cytomegalovirus promoter in the 5' long terminal repeat, an extended packaging signal for rapid production of high-titer retroviral particles, and many convenient cloning sites for cDNA library construction. The vesicular stomatitis vir...

Name of Sequences. Porcine micromolar calcium-activated neutral protease 1 isoforms A and B (CAPN1A, CAPN1B) cDNA. Genus and Species. Sus scrofa (Meishan ×White Composite). Origin of Clones. Primers (mu1725 and mu1840; Smith et al., 2000) developed from the complete cDNA sequence of bovine CAPN1 (GenBank accession no. AF221129) were used to screen one embryonic and one adult pooled tissue porci...

Estimation of population heterozygosity and library construction induced mutation rate from expressed sequence tag collections. Abstract: Unigene alignments obtained from cDNA libraries made using multiple individuals are not currently used to estimate population heterozygosity, as they are known to harbor mutations created during library construction. We describe an estimator of population het...

The normalization and subtraction of highly expressed cDNAs from relatively large tissues before cloning dramatically enhanced the gene discovery by sequencing for the mouse full-length cDNA encyclopedia, but these methods have not been suitable for limited RNA materials. To normalize and subtract full-length cDNA libraries derived from limited quantities of total RNA, here we report a method t...

We have developed a cDNA library screening method which allows the simultaneous screening of >10 ( 12 ) double-stranded plasmid cDNA molecules with minimal a priori sequence knowledge. A biotinylated, gene-specific oligonucleotide probe along with abutting 'blocking' oligos is hybridized to the plasmid cDNA library and the target plasmid retrieved with paramagnetic streptavidin beads and transf...