background: melon (cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. to date, two allergen molecules, cuc m 1 and cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of cuc m 3. methods:the cuc m 3 cdna has been characterized by rapid amplification of cdna ends (race), which rev...

Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. Methods:The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which ...

Abstract Serine protease inhibitors (serpins), a superfamily of inhibitors, are known to be involved in several physiological processes, such as development, metamorphosis, and innate immunity. In our study, full-length serpin cDNA, designated Haserpin1, was isolated from the cotton bollworm Helicoverpa armigera. The cDNA sequence Haserpin1 is 1176 nt long, with an open reading frame encoding 3...

The accurate evaluation of telomerase activity, a typical cancer biomarker, is vital for early screening. In this study, we developed dark-field microscopy (DFM) visual single-particle detection scheme to detect activity based on automatic counting gold nanoparticles (AuNPs). This method started with attaching the substrate (TS) primer magnetic beads (MBs) through streptavidin-biotin interactio...

PURPOSE Rods and cones differ in their photoresponse characteristics, morphology, and susceptibilities to certain diseases. To contribute to the studies at the molecular level of these differences, we tried to identify genes expressed preferentially in rods or cones. METHODS From purified carp rods and cones, we extracted their RNA and obtained corresponding cDNA pools (rod cDNA and cone cDNA...