BACKGROUND The management of peanut allergy relies on allergen avoidance and epinephrine autoinjector for rescue treatment in patients at risk of anaphylaxis. Biomarkers of severity and threshold of allergic reactions to peanut could significantly improve the care for patients with peanut allergy. OBJECTIVE We sought to assess the utility of the basophil activation test (BAT) to predict the s...

BACKGROUND AND PURPOSE Combined antiplatelet agents may offer additive protection over single drugs after stroke. We investigated whether platelet activation is reduced under combined aspirin and clopidogrel compared with each drug alone. METHODS In a case-crossover study, 31 patients with previous atherothrombotic or lacunar stroke who were treated with aspirin (100 to 300 mg/d) received clo...

AIM To evaluate platelet activation markers in psoriasis patients, compared to controls, and investigate their association with the inflammatory burden of psoriasis. METHODS Forty psoriatic patients without cardiovascular disease, and 12 healthy controls were subjected to measurement of baseline platelet CD62P, CD63 and CD42b expression, platelet-leukocyte complexes, i.e., platelet-monocyte c...

Cancer recognized as one of the leading irrepressible health issues is contributing to increasing mortality-rate day-by-day. The tumor microenvironment is an important field of cancer to understand the detection, treatment and prevention of cancer. Recently, cancer stem cell (CSC) research has shown promising results aiming towards cancer diagnostics and treatment. Here, we found that prostate ...

Abstract Despite their roles in intercellular communications, the different populations of extracellular vesicles (EVs) and secretion mechanisms are not fully characterized: how to what extent EVs form as intraluminal endocytic compartments (exosomes), or at plasma membrane (PM) (ectosomes) remains unclear. Here we follow intracellular trafficking EV markers CD9 CD63 from endoplasmic reticulum ...

Extracellular vesicles (EVs) can mediate intercellular communication, including signaling between the placenta and maternal tissues. Human placental explant culture is a versatile in vitro model system to investigate function. We performed systematic studies identify defined serum-free medium that supports high trophoblast viability metabolism, as well release of similar populations EVs, compar...

BACKGROUND Subcutaneous Immunotherapy (SCIT) modifies the allergic response and relieves allergic symptoms. SCIT is the only and a very effective treatment for insect venom allergy. We hypothesized that basophil sensitivity, measured through the basophil activation test, would decrease during SCIT up dosing. Expression of CD203c was compared to CD63 as marker for basophil activation, using a Bl...

The macrophage home of HIV H IV assembles in a compartment that lies deep within macro-phages yet is continuous with the extracellular environment, report Deneka et al. (page 329). HIV forms membrane-encapsulated particles that assemble at, and bud off from, the surface of infected T cells. In macro-phages, however, the majority of virus particles are found intracellularly and have been reporte...