نتایج جستجو برای: cccdna
تعداد نتایج: 249 فیلتر نتایج به سال:
In the replication cycle of hepadnavirus DNA, the double-stranded linear form of viral DNA is generated as a minor replicative intermediate, which is efficiently converted to covalently closed circular DNA (cccDNA) by intramolecular recombination (W. Yang and J. Summers, J. Virol. 69:4029-4036, 1995). We previously found a binding site of transcription factor Yin and Yang 1 (YY1) in one termina...
PURPOSE The combination of hepatitis B immunoglobulin (HBIg) and nucleos(t)ide analogues has been accepted as the best treatment to control hepatitis B recurrence after orthotopic liver transplantation (OLT). However, the optimal dose of HBIg remains unclear. We have previously reported that high-dose HBIg in the early period followed by low-dose HBIg with nucleos(t)ide analogues offers reliabl...
BACKGROUND A functional cure of chronic hepatitis B (CHB) is feasible, but a clear view of the intrahepatic viral dynamics in each patient is needed. Intrahepatic covalently closed circular DNA (cccDNA) is the stable form of the viral genome in infected cells, and represents the ideal marker of parenchymal colonization. Its relationships with easily accessible peripheral parameters need to be e...
with up to 400 million affected people worldwide, chronic hepatitis b virus (hbv) infection is still a major health care problem. during the last decade, several novel therapeutic approaches have been developed and evaluated. in most regions of the world, interferon-α (ifn-α), and nucleos(t)ide analogues are currently approved. despite major improvements, none of the existing therapies is optim...
results a plasmid construct, including sequences from the hbv genome and from the human gene htert, was generated as an isomolar multi-standard for hbv quantitation and normalization to the cellular contents. the specificity of the real-time assay for the cccdna was assessed using dane particles isolated on a density gradient. a comparison of liver tissue from 6 untreated and 6 treated patients...
One of the major problems in gaining further insight into hepatitis B virus (HBV)/host-cell interactions is to improve the existing cellular models for the study of HBV replication. The first objective of this study was to improve the system based on transduction of HepG2 cells with a recombinant baculovirus to study HBV replication. A new HBV recombinant baculovirus, Bac-HBV-1.1, in which the ...
The direct electrochemistry of hepatitis B virus containing the covalently closed circular DNA (HBV cccDNA) is realized at pretreated glassy carbon electrode (PGCE). It essential that electron transfer guanine (G) and adenine (A) on HBV cccDNA strand captured by microwave irradiation to melt into ssDNA. irreversible adsorption diffusion controlled processes G A are revealed cyclic voltammetry w...
Hepatitis B virus (HBV) core protein assembles viral pre-genomic (pg) RNA and DNA polymerase into nucleocapsids for reverse transcriptional DNA replication to take place. Several chemotypes of small molecules, including heteroaryldihydropyrimidines (HAPs) and sulfamoylbenzamides (SBAs), have been discovered to allosterically modulate core protein structure and consequentially alter the kinetics...
BACKGROUND Hepatitis B virus (HBV) DNA has been shown to contain CpG islands that are methylated in human tissue, which suggests a role for methylation in regulating viral protein production. However, data are lacking about whether methylation regulates viral gene expression. METHODS To investigate the hypothesis that methylation of viral DNA regulates viral gene expression, unmethylated, par...
Duck hepatitis B virus (DHBV) is a model virus for human hepatitis B virus (HBV), which infects approximately 360 million individuals worldwide. Nucleoside analogs can decrease virus production by inhibiting the viral polymerase; however, complete clearance by these drugs is not common because of the persistence of the HBV episome. HBV DNA is present in the nucleus as a covalently closed circul...
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