objective(s): protein kinase c (pkcα) is involved in modulating articular chondrocytes apoptosis induced by nitric oxide (no). hyaluronic acid (ha) inhibits nitric oxide-induced apoptosis of articular chondrocytes by protecting pkcα, but the mechanism remains unclear. the present study was performed to investigate the effects and mechanisms of pkcα regulate protective effect of hyaluronic acid....

Cytochrome c-mediated activation of caspase-3 is the final common pathway for most signals that induce apoptosis. Before release of cytochrome c from mitochondria, K(+) and Cl(-) efflux and intracellular acidification must occur. We have utilized an in vitro assay to examine the role of pH, cations, anions, and uncharged molecules on the process of cytochrome c-mediated activation of procaspase...

in this study organotypic adult spinal cord slices were used to investigate whether caspases could participate in the apoptosis of motor neurons. the thoracic region of spinal cord was sliced using a tissue chopper and cultured in a medium for 6h. morphological and biochemical features of apoptosis were assessed by fluorescent staining and terminal deoxynucleotidyl nick end labeling (tunel) met...

The purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-CdA) in the human breast cancer cell line, MDA-MB468 (estrogen receptor negative, ER−). MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide] assay, annexin V-Fluorescein/PI and Hoechst 33258 staining were used to detect cytotoxicity and cell apopto...