Alizarin red, confocal laser scanning microscopy, dentinal tubules, human caries. Abstract Dentin morphology and the lesion found in dental caries have been studied for many years. It was first observed under optical microscopy, and later using electron microscopy. Confocal laser scanning microscopy (CLSM) applied with several fluorescent dyes such as alizarin red to see normal dentinal tubules...

Although there are several studies that have evaluated the bond strength of various adhesives to healthy dentin and caries-affected after traditional caries removal, objective this systematic review aimed assess (CAD) chemo-mechanical removal (CMCR) treatment. The adhered PROSPERO protocol registration followed PRISMA guidelines. research question focused on bonding effectiveness dental CAD emp...

The aim of this work was to verify the existence of correlation between Raman spectroscopy readings of phosphate apatite (~960 cm-1), fluoridated apatite (~575 cm-1) and organic matrix (~1450 cm-1) levels and Diagnodent® readings at different stages of dental caries in extracted human teeth. The mean peak value of fluorescence in the carious area was recorded and teeth were divided in enamel ca...

Hidden caries is a type of lesion visualized in dentin, mainly by means of bitewing radiographs, in which, clinically, the occlusal enamel appears healthy or minimally demineralized and radiographically presents progressive demineralization in dentin, which may progress and compromise the pulp-dentin complex. Although the etiology of hidden caries is unknown, many theories have been studied, in...

BACKGROUND Identifying the microbial species in caries lesions is instrumental to determine the etiology of dental caries. However, a significant proportion of bacteria in carious lesions have not been cultured, and the use of molecular methods has been limited to DNA-based approaches, which detect both active and inactive or dead microorganisms. OBJECTIVE To identify the RNA-based, metabolic...

Arresting occlusal enamel caries lesions with pit and fissure sealants The aim of this study was to evaluate the feasibility of pit and fissure sealants and the effectiveness of the two sealant methods applied in every-day practice in public dental health care in Finland. Two sealant methods were evaluated according to their effectiveness in preventing dentin caries at occlusal surfaces and sea...

PURPOSE The purpose of this in vivo study of primary teeth was to analyze the ultrastructure and microbiology of dentin layers affected by caries lesions before and after restorations with resin-modified glass ionomer. METHODS Samples of carious dentin from primary teeth removed prior to restoration placement (baseline-0 day) were compared with samples taken after 30 and 60 days. Dentin from ...