Methyl tert-butyl ether (MTBE), an octane enhancer and a fuel oxygenate in reformulated gasoline, has received increasing public attention after it was detected as a major contaminant of water resources. Although several techniques have been developed to remediate MTBE-contaminated sites, the fate of MTBE is mainly dependent upon natural degradation processes. Compound-specific stable isotope a...

The sulfur isotope effect produced by sulfate reducing microbes is commonly used to trace biogeochemical cycles of sulfur and carbon in aquatic and sedimentary environments. To test the contribution of intracellular coupling between carbon and sulfur metabolisms to the overall magnitude of the sulfur isotope effect, this study compared sulfur isotope fractionations by mutants of Desulfovibrio v...

Dramatic warming and upheaval of the carbon system at the end of the Paleocene Epoch have been linked to massive dissociation of sedimentary methane hydrate. However, testing the Paleocene-Eocene thermal maximum hydrate dissociation hypothesis has been hindered by the inability of available proxy records to resolve the initial sequence of events. The cause of the Paleocene-Eocene thermal maximu...

Fifty-eight dental calculus samples from medieval and post-medieval skeletons from Vitoria, Spain, and a single sample from an Alaskan Inuit were tested for stable carbon and nitrogen isotope compositions. There was sufficient carbon and nitrogen concentrations to obtain d13C and d15N values, and the samples from Spain produced results that were replicable and comparable to European isotope val...

Marine stable isotope records provide the basis for much of our understanding of past climates. During the past four decades of research, the exploitation of climatic information contained in marine stable isotopes led to the generation of a global network of marine stable isotope records. In particular, oxygen isotope records have been used to estimate past water temperatures, ice sheet sizes,...

A series of laboratory studies were conducted to increase understanding of stable carbon (C/C) and hydrogen (D/H) isotope fractionation arising from methanogenesis by moderately thermophilic acetateand hydrogen-consuming methanogens. Studies of the aceticlastic reaction were conducted with two closely related strains of Methanosaeta thermophila. Results demonstrate a carbon isotope fractionatio...

The stable carbon isotope (13)C is used as a universal tracer in plant eco-physiology and studies of carbon exchange between vegetation and atmosphere. Photosynthesis fractionates against (13)CO(2) so that source sugars (photosynthates) are on average (13)C depleted by 20‰ compared with atmospheric CO(2). The carbon isotope distribution within sugars has been shown to be heterogeneous, with rel...

Contaminant carbon (blank carbon) was studied for its impact on the carbon isotope measurements (∆14C and δ13C) of 3 organic compound classes of oceanic particulate organic matter. Two methods of blank correction and associated uncertainties were studied. First, the carbon blanks were quantified manometrically and the isotope ratios of the blank carbon were measured directly. Second, the isotop...

The mechanistic understanding of isotope fractionation processes is increasing but we still lack detailed knowledge of the processes that determine the isotopic composition of the tree-ring archive over the long term. Especially with regard to the path from leaf photosynthate production to wood formation, post-assimilation fractionations/processes might cause at least a partial decoupling betwe...

RATIONALE Compound-specific isotope analysis (CSIA) relies on continuous flow combustion of organic substances to CO(2) and N(2) in a miniature reactor to measure (13)C/(12)C and (15)N/(14) N stable isotope ratios. Accurate analysis is well established for many volatile hydrocarbons. In contrast, compounds which contain hetero and halogen atoms are less volatile and may be more recalcitrant to ...