Immature embryos as a choice tissue for genetic transformation of maize have a few limitations, such as genotype dependence, time-consuming and requiring a well-equipped greenhouse for access, at any time. In the present study, the split-seed explants were used for genetic transformation of maize, B73 line. The transformation of maize split-seed explants from the inbred line B73, for resistance...

A widely applicable promoter system is described that allows a gene of interest to be activated in specific plant tissues after a cross between defined transgenic lines. The promoter, pOp, consists of lac operators cloned upstream of a minimal promoter. No expression was detected from this promoter when placed upstream of a beta-glucuronidase (GUS) reporter gene in transgenic plants. Transcript...

The plastid psbA promoter of tobacco was used with the aim to construct plastid specific marker genes. Upon transfer to the tobacco nuclear genome the plastid promoter fragment appeared to specify a messenger RNA. Placed 5' to the bar or nptII coding sequences the level of expression is sufficient to obtain a selectable phenotype. The transcription start site in the nucleus is site specific and...

artemisia annua is an annual herb native to asia most probably china produce one of the most important sesquiterpene namely artemisinin which has antimalarial and anticancer activity. three transcription factors belonging to wrky family, two transcription factors belonging to myb family and two enzymes dbr2 and opr3 were isolated from a.  annua leaves and first inserted into pjet1.2 vector then...

We have shown previously that gene VI of cauliflower mosaic virus (CaMV) strain D4 governs systemic infection of Nicotiana bigelovii and that transgenic N. bigelovii expressing the D4 gene VI product can complement at least one CaMV isolate for long-distance transport. We have now found that DNA of two other isolates of CaMV recombine with the gene VI coding sequence present in the transgenic p...

The detection and exact quantification of the presence of GMOs (genetically modified organisms, also named as living modified organisms, LMOs) grains has become very important in international commercial transactions, especially from countries producing both types of commodities, GMOs and GMO-free. This makes necessary to check every batch previous delivery to the recipient country. Several PCR...

Two bacterial antibiotic resistance genes, one coding for the neomycin phosphotransferase (NPT I) from Tn903, and the other coding for the chloramphenicol acetyltransferase from Tn9 were used as plant selectable markers. Both genes were introduced into the Nicotiana tabacum genome in a new plant expression vector, using the direct gene transfer method. The vector pDH51, used in these experiment...

Objectives: (1) Obtain a genomic clone of the apple α-FS gene including the promoter and ethylene response element(s); (2) Isolate and characterize the promoter region from the genomic clone of the apple peel HMG2 gene and determine whether expression of HMG2 is regulated by ethylene; (3) Evaluate the correlation of ethylene-induced transcription of genes encoding enzymes in the αfarnesene synt...