establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites for genetic transformation of any crop plant. in vitro culture of lentil has proven to be difficult. in spite of a number of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet been reported. this study carried out for investigation of different hor...

Teucrium chamaedrys is regarded as an herbaceous perennial plant from Lamiaceae family that is used as medicinal plants and food from ancient times. Due to the recent developments in tissue culture techniques and extraction of secondary metabolites, this study aimed to investigate the explants of the Teucrium for callus induction and secondary metabolites. The interaction of Auxin hormones incl...

Obtainment and characterization of the novel endosperm callus of Taxus chinensis Rehd. var. mairei are valuable for haploid breeding, genome, and functional genome in Taxus. Callus was obtained by hydropriming with sterile water for 3 days and suitable medium composition. The highest callus induction (70.89 %) and lower browning ratio (7.95 %) were obtained from Gamborg (B5) medium supplemented...

Effects of different concentrations of IAA (0, 0.3, 0.6 mg/l) and 2ip (0, 0.3, 0.6, 0.9, 1.2 mg/l) and their combinations on callus induction and shoot regeneration of hypocotyl and its thin cell layer (TCL) explants in tomato was studied. Explants were prepared from hypocotyls of seedlings in the aseptic condition. Hypocotyl segments were more efficient than TCL explants for callus induction a...

parsi zira, bunium persicum (boiss.) b. fedtsch., or black zira, a member of the umbellifera family, is one of the most important medicinal species with high economic value. generally, there is limited information about in vitro culture of bunium persicum. in this study, the effect of various media (ms, b5) and combinations of plant growth regulators, naa and 2,4-d alone or together with kin, o...

The induction efficiency of maize embryonic callus is highly dependent on the genotype, and only a few lines possess a high capacity for callus formation. Although certain genes and pathways have been reported to contribute to the regulation of callus induction, to the best of our knowledge, the functions of the small interfering RNAs (siRNAs) involved in this process remain unknown. In this st...

In present report effects of explants type, basal media and plant growth regulators (PGRs) were tested for induction of indirect somatic embryogenesis in medicinally important plant Silybum marianum L. Leaf, petiole and root explants were exploited in vitro on B5 (Gamborg), SH (Schenk and Hildebrandt) and MS (Murashige and Skoog) media for induction of embryogenic callus followed by somatic emb...

Stevia rebaudiana Bertoni that conventionally propagated by seed or by cuttings or clump division which has a limitation of quality and quantity seed material. In present study, callus culture technique was tried to achieve rapid plant multiplication for quality seed material. Callus induction and multiplication medium was standardized from nodal as well as leaf sagments. It is possible to main...

Differentiation and morphogenetic potential of Allium commutatum Guss. callus tissue was investigated in five subcultures. Callus tissue was derived from root tips of in vitro grown seedlings and maintained on MS medium supplemented with 3% sucrose, 0.8% agar, 4.5 pM 2,4-D and 4.6 pM kinetin. Pieces of calli were subcultured on media with different concentrations and combinations of 2,4-D (1.0,...

Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg x l(-1) kinetin, and three levels of 2,4-D. The highest callus relative growth was obta...