Stevia rebaudiana (Bert.) Bertoni, commonly called “sweet leaf” is a medicinally and industrially important plant known to be rich in zero-calorie natural sweetening compound(s) as “steviol glycosides”. However, due its poor seed germination slow vegetative propagation, it has become rather difficult meet the increasing global demand for Stevia-based products. Different biotechnological approac...

In order to select the best tomato cultivar for Agrobacterium mediated gene transformation studies, In vitro regeneration frequency of hypocotyls, leaf disc and shoot tip of five tomato cultivars (Lycopersicon esculentum) was investigated on a regeneration medium supplemented with 1 mg/l zeatin and 0.1 mg/l indole-3-acetic acid. Significant differences in regeneration capacity between genotypes...

BACKGROUND Human granulocyte colony-stimulating factor (hG-CSF) can serve as valuable biopharmaceutical for research and treatment of the human blood cancer. Transplastomic plants have been emerged as a new and high potential candidate for production of recombinant biopharmaceutical proteins in comparison with transgenic plants due to extremely high level expression, biosafety and many other ad...

Somatic embryogenesis has been accomplished from tender leaf base explant of date palm (Phoenix dactylifera L.). Three to five mm long tender leaf base explants derived from the meristamatic region of 2-3 year old offshoots of date palm cv. Kheneizi were cultured on Murashige and Skoog (MS) basal medium supplemented with 10, 50, 100 and 150 mg l 2,4-dichlorophenoxy acetic acid (2,4-D) and incub...

The present work describes an efficient micropropagation and callus induction protocol of Limbarda crithmoïdes L. (Asteraceae), a halophyte species with medicinal horticultural interests. objective was to identify the culture media that support best organogenesis callogenic expressions four types aerial explants; nodal internodal segments leaf portions, by varying mineral composition twenty add...

Abstract Pranajiwa (Euchresta horsfieldii (Lesch) Benn.) is an endangered medicinal plant known for its function as aphrodisiac, antidote, expectorant, and tonic. It also can be used to cure TBC. Research has found that pranajiwa difficult propagated, but the tissue culture propagation method never been applied this species. This research aimed figure out procedure induce callogenesis of with a...

A simple micropropagation protocol has been achieved using nodal region and apical leaf of Clerodendrum indicum,(L) kuntze; an important medicinal plant of Verbenaceae. The explants were cultured in MS medium supplemented with NAA, BA, 2,4-D,Kn, IBA in various combination and concentrations (0.110.0 μM). Response to callogenesis were diverse, ranging from white vigorous compact callus (1.0μM NA...

Clonal reproduction of oil palm by means of tissue culture is a very inefficient process. Tissue culturability is known to be genotype dependent with some genotypes being more amenable to tissue culture than others. In this study, genetic linkage maps enriched with simple sequence repeat (SSR) markers were developed for dura (ENL48) and pisifera (ML161), the two fruit forms of oil palm, Elaeis ...