Bovine and caprine Monterey Jack cheeses were produced unfortified (cheese) or fortified with milk calcium (Ca-cheese). Five groups of male rats were fed a control diet or one of four experimental diets: bovine cheese diet, bovine Ca-cheese diet, caprine cheese diet, and caprine Ca-cheese diet to test their effects on calcium absorption as well as bone mineralization. Significant differences (P...

1. The release of catecholamines (CA) from rat adrenal incubated in vivo in Locke solution was studied. 2. Acetylcholine-induced release of CA and CA release by 56 mM KCl were inhibited by verapamil and lanthanum chloride which block calcium permeability. 3. CA secretion induced by salbutamol or by theophylline was unaffected by either verapamil or lanthanum chloride. 4. Dantrolene-sodium inhib...

AIMS The requirement of endothelial NO synthase (NOS3) calcium to produce NO is well described, although the effect of NO on intracellular calcium levels [Ca(2+)](i) is still confusing. Therefore, NO and [Ca(2+)](i) cross-talk were studied in parallel in endothelial cells possessing a functional or a dysfunctional NO pathway. METHODS AND RESULTS Dysfunctional porcine endothelial cells were ob...

OBJECTIVE To investigate the effects of stimulating calcium induced Ca release with low concentrations (100-200 microM) of caffeine and, in particular, to study the cellular mechanisms responsible for the transient responses found previously. METHODS Experiments were performed on isolated rat ventricular myocytes. Intracellular calcium concentration ([Ca2+]i) was measured with Indo-1, the cel...

the aim of this study was to investigate the sol gel synthesis of ha/fa nanoparticles and the possible formation of tcp phase or unstoichiometric calcium deficient hydroxyapatite (cdha) from the precursors with a ca/p ratio of 1.62. in order to prepare the sol, the solutions of triethyl phosphite, ammonium fluoride and calcium nitrate in ethanol were used respectively as p, f and ca precursors....

A new method for the measurement of calcium isotope ratios and total calcium, using a double focusing sector field inductively coupled plasma mass spectrometry (ICP-MS) instrument equipped with a shielded torch, is presented. The method was applied for the measurement of the Ca/Ca, Ca/Ca and Ca/Ca ratios and total calcium by isotope dilution in urine samples from two different nutritional exper...

During the past decades, many different fluorescent indicators have been developed for measuring intracellular ion concentrations. Of particular interest are fluorescent calcium indicators because of the fundamental role of Ca in various cellular processes such as contraction, secretion, and gene activation. For a quantitative understanding of the physiological roles of Ca, fluorescence signals...

In this study we analyzed the effect of 4-aminopyridine (4-AP) on free cytosolic calcium concentration ([Ca(2+)](i)) in basal conditions, after stimulation with neurotransmitters, and during capacitative calcium entry. Using fura-2 ratiometric calcium imaging, we found that 4-AP increased [Ca(2+)](i) in type I astrocytes, neurons, and in skeletal muscle cells. The [Ca(2+)](i) elevation induced ...

Blood fl ow is intimately linked to endothelial membrane potential and intracellular Ca 2+ levels ([Ca 2+ ] i ). Endothelial Ca 2+ infl ux appears to depend on the electrochemical gradient, and likely occurs through nonvoltage-dependent Ca 2+ entry pathways, possibly transient receptor potential (TRP) channels. Therefore, hyperpolarization of the endothelium membrane elevates [Ca 2+ ] i by an i...

Mitochondrial calcium plays a crucial role in mitochondrial metabolism, cell calcium handling, and cell death. However, some mechanisms concerning mitochondrial calcium regulation are still unknown, especially how mitochondrial calcium couples with cytosolic calcium. In this work, we constructed a novel mitochondrial calcium fluorescent indicator (mito-GCaMP2) by genetic manipulation. Mito-GCaM...