Murphy, Jason G., Jason N. Herrington, Joey P. Granger, and Raouf A. Khalil. Enhanced [Ca2 ]i in renal arterial smooth muscle cells of pregnant rats with reduced uterine perfusion pressure. Am J Physiol Heart Circ Physiol 284: H393–H403, 2003. First published September 12, 2002; 10.1152/ajpheart.00247.2002.—Reduction of uterine perfusion pressure (RUPP) during late pregnancy has been suggested ...

Neurons of the avian cochlear nucleus, nucleus magnocellularis (NM), are stimulated by glutamate, released from the auditory nerve, and GABA, released from both interneurons surrounding NM and from cells located in the superior olivary nucleus. In this study, the Ca2+ indicator dye Fura-2 was used to measure Ca2+ responses in NM stimulated by glutamate- and GABA-receptor agonists using a chicke...

Changes in intracellular free calcium concentration ([Ca2+]i) are known to influence a variety of events in developing neurons. Although spontaneous changes of [Ca2+]i have been examined in immature cortical neurons, the calcium dynamics of cortical precursor cells have received less attention. Using an intact cortical mantle and confocal laser microscopy, we examined the spatiotemporal pattern...

Entamoeba histolytica adherence and destruction of host cells is required for in vivo pathogenicity; amebic in vitro adherence is mediated by a galactose- or N-acetyl-D-galactosamine-inhibitable surface lectin (Gal/GalNAc adherence lectin). Free intracellular Ca2+ concentration [( Ca2+]i) was measured in living amebae and target cells during amebic cytolysis of Chinese hamster ovary (CHO) cells...

Yakubu, Momoh A., and Charles W. Leffler. L-type voltage-dependent Ca2 channels in cerebral microvascular endothelial cells and ET-1 biosynthesis. Am J Physiol Cell Physiol 283: C1687–C1695, 2002. First published August 22, 2002; 10.1152/ajpcell.00071.2002.—We investigated the role of intracellular calcium concentration ([Ca2 ]i) in endothelin-1 (ET-1) production, the effects of potential vasos...

Monensin, a Na(+)-selective ionophore, enhances transmitter release when applied to crustacean and frog neuromuscular junctions. Monensin is believed to raise intracellular sodium ([Na+]i) which in turn elevates intracellular calcium ([Ca2+]i). Using the fluorescent indicator fura-2, we measured [Ca2+]i in crayfish Procambarus clarkii presynaptic terminals during monensin application in normal ...

The [Ca2+]i of individual neurosecretory nerve terminals loaded with the fluorescent probe fura-2 was monitored during depolarizing stimuli and in the presence of substances known to induce or block neurohormone release. Induction of membrane depolarization with elevated [K+] or veratridine led to a rapid increase in [Ca2+]i that was sensitive to block by substances which block voltage-sensitiv...

PURPOSE Subcellular Ca2+ signaling patterns, such as Ca2+ waves, gradients, and oscillations, are an important aspect of cell regulation, but the molecular basis for these signaling patterns is not understood. Because Ca2+ release patterns differ among isoforms of the inositol 1,4,5-trisphosphate (InsP3) receptor, the relationship between the distribution of these isoforms and subcellular Ca2+ ...

The contractile force generated by hepatic stellate cells in response to endothelin-1 contributes to sinusoidal blood flow regulation and hepatic fibrosis. This study's aim was to directly test the widely held view that changes in cytosolic Ca2+ concentration ([Ca2+]i) mediate stellate cell force generation. Contractile force generation by primary cultures of rat hepatic stellate cells grown in...

Osteoclasts are multinucleated, bone-resorbing cells that show structural and functional differences between the resorbing and nonresorbing (motile) states during the bone resorption cycle. In the present study, we measured intracellular Ca2+ concentration ([Ca2+]i) in nonresorbing vs. resorbing rat osteoclasts. Basal [Ca2+]i in osteoclasts possessing pseudopodia (nonresorbing/motile state) was...