background previous studies using cell culture systems for the replication of hepatitis c virus have opened new research dimensions, and paved the ways for further and detailed studies of the virus in vitro. objectives the purpose of the present study was to cultivate hepatitis c virus in a cell culture system and evaluate viral amplification. materials and methods in order to propagate hepatit...

introduction: in this study we examined the effect of ampullary and isthmic epithelial cell of golden hamster oviduct on n-mary mouse embryo development during 4-days in vitro co - culture, and the effect of injected gonadotropins on the quality of co-culture. materials and methods: golden hamster oviducts were isolated 18-24h after treating the hamster with hcg. they were flushed with dmem / h...

Understanding the initial events in the establishment of vaginal human immunodeficiency virus type-1 (HIV-1) entry and infection has been hampered by the lack of appropriate experimental models. Here, we show in an ex vivo human organ culture system that upon contact in situ, HIV-1 rapidly penetrated both intraepithelial vaginal Langerhans and CD4(+) T cells. HIV-1 entered CD4(+) T cells almost...

Background: The aim of the present study was to develop a three-dimensional (3D) collagen gel culture system for the in vitro growth and survival of buffalo preantral follicles with or without growth factors. Materials and Methods: Buffalo ovaries were collected from a local abattoir and preantral follicles were isolated through microdissection. Isolated preantral follicles were put either in c...

Cultivation of Hotate clams (Mizuhopecten yessoensis) in Funka Bay, Hokkaido, Japan, has become an attractive option for farmers. This study assessed the feasibility clam culture sites Japan based on water quality data such as temperature, salinity and depth. The analysis showed that site is highly suitable culture, with temperature ranging from 11.5°C to 22.3°C, 31.01 32.97 psu, depth up 25 me...