We developed a method, named GraFix, that considerably improves sample quality for structure determination by singleparticle electron cryomicroscopy (cryo-EM). GraFix uses a glycerol gradient centrifugation step in which the complexes are centrifuged into an increasing concentration of a chemical fixation reagent to prevent aggregation and to stabilize individual macromolecules. The method can ...

Current findings suggest a role for the angiotensin II (Ang II) signalling pathway in generation of reactive oxygen species and diabetes-induced cardiac complications. In this study we aimed to investigate the effect of angiotensin II type 1 (AT 1 ) receptor blockage on some antioxidant enzy mes such as glucose-6-phosphate dehydrogenase (G6PD), 6-phoshogluconate dehydrogenase (6PGD), glutathion...

Meal machine, experimental laboratory, status symbol, domestic prison, or the creative and spiritual heart of the home? Over the course of the past century no other room has been the focus of such intensive aesthetic and technological innovation, or as loaded with cultural significance. Kitchen design has been both a central concern of modernism and fundamental to our concept of modern life (Mo...

IN RECENT YEARS, THERE HAS BEEN A RAPID AND SIGNIFICANT PROGRESS IN UNDERSTANDING THE MOLECULAR BASIS OF THE CIRCADIAN RHYTHMICITY, AS SEVERAL CLOCK GENES HAVE BEEN CLONED IN MAMMALS. Studies with these genes focusing on loss of function have demonstrated abnormal sleep/wake length periods, disturbed entrainment, and loss of persistence of circadian rhythms. Current findings clearly indicate th...

Ohmic heating (OH) was evaluated as a post-harvest technology to improve chestnuts’ shelf-life (Castanea sativa Mill.) by controlling molds and insect larvae proliferation. Chestnuts were processed OH at 35, 45, 55 °C compared with untreated fruits the conventional hydrothermal (HT - 50 for 45 min), which is process currently used chestnut industry. Shelf-life studies carried out different atmo...

More than eighty percent of patients with coronary heart diseases (CHD) have conventional risk factors. Prevalence of well known risk factors seems to show a different pattern in younger patients and individual above 55 years. To evaluate the pattern of conventional CHD risk factors in healthy individuals in two different age groups. A large scale population based survey of 31999 individuals fr...

This paper reports the results for the pH of three buffer solutions free of chloride ion. The remaining six buffer solutions have saline media of the ionic strength I = 0.16 mol·kg, matching closely to that of the physiological sample. Conventional paH values for the three buffer solutions without the chloride ion and six buffer solutions with the chloride ion at I = 0.16 mol·kg from 5 ̊C to 55 ̊...

Background: Drying is the most common way to preserve quality of aromatic and medicinal plants. Chosen drying methods can affect on the essential oil content and composition of medicinal plants. Objective: This study aimed to assess the changes in essential oil constitutes of catnip aerial part under various drying methods. Methods: The experiment was performed as the randomized complete ...

Abstract The high antioxidant capacity of tea is well-known, but the effect flavorings like honey or lemon has been less studied. Their antioxidants can interact with each other, global result being also affected by brewing temperature. combined heat (55 and 80 °C) (acacia honeydew honeys, juice) on total polyphenol, flavonoid content black green teas was In many cases higher obtained at °C. Te...

The cation distribution of the NTC thermistor spinel N i M n 2 O 4 $NiM{n}_2{O}_4$ was studied in temperature range from 55°C to 900°C, using a combined in-situ neutron diffraction and thermopower study. Rietveld refinements data reveal dependence degree inversion with an parameter 0.70(1) at 900°C 0.87(1) 55°C. Thermopower measurements were evaluated modified Heikes formula [Mn4+]/[Mn3+]-ratio...