Spliced leader trans-splicing occurs in many primitive eukaryotes including nematodes. Most of our knowledge of trans-splicing in nematodes stems from the model organism Caenorhabditis elegans and relatives, and from work with Ascaris. Our investigation of spliced leader trans-splicing in distantly related Dorylaimia nematodes indicates that spliced-leader trans-splicing arose before the nemato...

results significant differences were observed in the results of co-culture of nematodes larva and fungi after seven days. the most effective fungi against the nematodes larvae were cladosporium sp., trichoderma sp., fusarium equisetti, after seven days of incubation. materials and methods sheep feces were employed to provide the required third stage larvae source for the experiments. the nemato...

From 1990 through 1992, nematodes were extracted from soil samples taken from the rhizosphere of native species of grapes from four areas of northern California and two areas of southern California. For comparison, samples from domestic grapes as well as a putative hybrid of Vitis califarnica and V. vinifera were also taken. Rhizosoil from California native grapevine contained many more species...

A checklist of nematode parasites from Indonesian murids with their geographic distribution is presented. This checklist is compiled from three sources: the catalogue of nematode parasites of Museum Zoologicum Bogoriense (unpublished specimens in the collection), data from our previous research and articles on nematodes of Indonesian murids. This checklist is presented as a list of nematode par...

The feasibility of counting plant-parasitic nematodes in aqueous suspensions by measuring light transmittance through aqueous suspensions with an ELISA microplate reader was explored. Absorbance readings for eggs or vermiform stages of three species were linearly related (R(2) > 0.99) to concentrations between 0 and 10,000 nematodes/ml. Coefficients of variation ranged from 12-23%, depending on...

A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic ne...

Soils and roots of field crops in low-rainfall regions of the Pacific Northwest were surveyed for populations of plantparasitic and non-plant-parasitic nematodes. Lesion nematodes (Pratylenchus species) were recovered from 123 of 130 non-irrigated and 18 of 18 irrigated fields. Pratylenchus neglectus was more prevalent than P. thornei, but mixed populations were common. Population densities in ...

Free-living nematodes are well-recognized as an abundant and ubiquitous component of benthic communities in inland waters. Compelling evidence from soil and marine ecosystems has highlighted the importance of nematodes as trophic intermediaries between microbial production and higher trophic levels. However, the paucity of empirical evidence of their role in freshwater ecosystems has hampered t...

In biological control programmes introduced natural enemies compete with indigenous enemies for hosts and may also engage in intraguild predation when two species competing for the same prey attack and consume one another. The large pine weevil, Hylobius abietis L. (Coleoptera: Curculionidae), is an important pest of coniferous reforestation in Europe. Among its natural enemies, the parasitoid ...

Four experiments were conducted using nematode-infested and nematode-free adults of the cerambycid beetle, Monochamus alternatus, to determine horizontal transmission pathways of Bursaphelenchus xylophilus. When nematode-infested beetles of one sex and nematode-free beetles of the opposite sex were paired in containers for 48 or 72 hours, the number of nematodes carried by nematode-free beetles...