Cryopreservation of oocytes and embryos is a supplementary technique for in vivo vitro embryo production. In the last decade, has been improved but did not achieve satisfactory commercial level. Also, results were variable. The pregnancy live birth rates 16 to 52% 5.3 26.9% after implantation vitrified embryos, respectively (from year 1993 2020). final consideration, quality most important fact...

(SCNT) embryos of buffaloes. 2. To study gene expression profile of important developmental genes at different stages of SCNT cloned embryo. 3. To study epigenetic reprogramming during early developments of SCNT embryos Materials and Methods: Expression analysis of developmental genes was done in different (ovarian granulose and cumulus and skin fibroblasts) donor cells; in vitro maturing oocyt...

Two experiments (Exp) were carried out to test the effect of different media on in vitro maturation (IVM) (Exp 1; n = 1142 ovaries with 2666 oocytes) and to study in vitro fertilization (IVF) of buffalo oocytes (Exp 2; n = 110 ovaries with 330 oocytes). In exp 1 the collected cumulus oocyte complex (COCs) was allocated to three maturational media; TCM-199 supplemented with hormones (M1), TCM-19...

Failure of male pronucleus formation has hampered the success of intracytoplasmic sperm injection (ICSI) in swamp buffalo. The aim of the present study was to improve male pronucleus formation by pretreating sperm with various chemicals before ICSI. In Experiments1 and 2, sperm were treated according to one of the following protocols: (1) 0.1% Triton-X 100 (TX) for 1 min, (2) 10 μM calcium iono...