background: sarcocystosis in south american camelids (sac) is an important parasitic disease which results in economical loss due to carcass condemnation. meat products from camelids are significant source of animal protein in several american countries. sarcocystis spp. producing macroscopical cysts in these animals have been nominated as s. aucheniae, s. tilopodi, and s. guanicoecanis. the ai...

background: dna isolation procedure can significantly influence the quantification of dna by real time pcr specially when cell free dna (cfdna) is the subject. to assess the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and to avoid problems with fragment size relevant to cfdna, development of appropriate external dna control (edc) is challenging. ...

objective(s) this study planned to assess the value of pcr is6110 assay in tissue specimens of needle pleural biopsy in patients suspicious to pleural tuberculosis. materials and methods sixty eight patients with lymphocytic exudative pleural effusion underwent pleural biopsy. tissue samples were sent for pathologic examination and pcr is6110 assay. the results of pcr reported as positive/ nega...

three tuberculous, twenty-one non-tuberculous mycobacterial (ntm) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of dna fragments in a polymerase chain reaction with single universal primer (up-pcr). a rather wide limit of tolerance for variations in procedure of pcr mixture preparation and thermocycling parameters was found. there...

The belly spot and tail (Bst(+/-)) mouse phenotype is caused by mutations of the ribosomal protein L24 (Rpl24). Among various phenotypes in Bst(+/-) mice, the most interesting are its retinal abnormalities, consisting of delayed closure of choroid fissures, decreased ganglion cells and subretinal vascularization. We further characterized the Bst(+/-) mouse and investigated the underlying molecu...

The objective of this study was to determine the minimum dosage of recombinant bovine somatotropin (bST) required to elicit maximum depression in plasma urea nitrogen (PUN), an indicator of anabolic activity. Twenty-four steers (389 kg) were blocked by weight into six pens. Six steers were placed on each of the following bST doses: 0, 8, 16 and 32 mg bST/d. Treatments were administered once dai...

In this study, we report a novel isothermal nucleic acid amplification method only requires one pair of primers and one enzyme, termed Polymerase Spiral Reaction (PSR) with high specificity, efficiency, and rapidity under isothermal condition. The recombinant plasmid of blaNDM-1 was imported to Escherichia coli BL21, and selected as the microbial target. PSR method employs a Bst DNA polymerase ...

background and objective: to estimate the risk of human papilloma virus (hpv) infection for cervical malignancies, we conducted a case-control study in southern iran (hormozgan province). materials and methods: for this purpose,52 paraffin embedded blocks with exact diagnosis of cervical carcinoma(50 carcinomas and 2 carcinomas in situ) from 2001 to 2006 and 52 praffin embedded blocks of cervic...