The identification of Brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates. The assay was designed in a multiplex format that will allow the rapid identification of Brucella spp., B. abortus, and B. melitensis in a single test.

Background: Virulence of pathogenic bacteria is often determined by their ability to adapt to stress. Result: The Brucella abortus general stress response (GSR) system is required for chronic mammalian infection, and is regulated by phosphorylation and proteolysis. Conclusion: The B. abortus GSR signaling pathway has multiple layers of post-translational control, and is a determinant of chronic...

Brucellosis is a zoonotic disease caused by Brucella sp. and may affect many parts of the body. Brucella epididymo-orchitis had been reported in up to 20% of patients with brucellosis. This is a case report of Brucella epididymo-orchitis in a Saudi male patient. He presented with a unilateral swelling of the left testicle. He had fever, arthralgia and night sweats. Ultrasound examination reveal...

OBJECTIVE 1) To identify patients of brucella spondylitis and sacroiliitis amongst patients of 'infective spondylitis' and 'sacroiliitis', 2) To study clinical and radiographic features and outcome of brucella spondylitis. METHODS We reviewed 21 patients of infective spondylitis and 12 of sacroiliitis attending our hospital between March and September 2000 and followed them to identify patien...

Aim: To investigate the preventive and therapeutic potential of brucella phage in experimentally infected mice with Brucella abortus (strain 544). Materials and Methods: Three groups of mice each containing 6 individuals were infected with Brucella abortus (strain 544). A preventive group was infected with Brucella abortus (strain 544), 48 hrs before phage treatment. A therapeutic group was pha...

The objective of this study was to determine seroprevalence of small ruminant lentivirus (SRLV), Brucella abortus and Brucella melitensis infections among dairy goats in South Backa and Srem region in Vojvodina Province. The other aim was to determine the risk factors associated with SRLV infection in investigated herds. A total of 971 serum samples were collected from 8 comercial dairy goat he...

Brucella spp. are intramacrophage pathogens that induce chronic infections in a wide range of mammals, including domestic animals and humans. Therefore, the macrophage response to infection has important consequences for both the survival of phagocytosed bacteria and the further development of host immunity. However, very little is known about the macrophage cell signaling pathways initiated up...

BACKGROUND Brucellosis is among the most widespread zoonotic infections estimated at 14% in Uganda. A cross-sectional study was conducted to estimate the sero-prevalence, risk factors of Brucella infection and malaria among abattoir workers. METHODS A survey was conducted among 232 abattoir workers in main abattoirs of Kampala and Mbarara districts in February 2007. A pre-tested questionnaire...

Brucellosis is a zoonotic and contagious infectious disease caused by infection with Brucella species. The infecting brucellae are capable of causing a devastating multi-organ disease in humans with serious health complications. The pathogenesis of Brucella infection is influenced largely by host factors, Brucella species/strain, and the ability of invading brucellae to survive and replicate wi...

introduction granulomatous epididymo-orchitis is a rare complication of brucellosis that usually appears in patients with antecedent brucella infection. case presentation the current study reports an 18-year-old man with acute epididymo-orchitis. in this case, an anti-brucella treatment was started based on epidemiological history and clinical findings, but the final diagnosis was established o...