Objective(s): The primary cytotoxic effects of anticancer drugs like idarubicin, a chemotherapeutic agent, are not limited to neoplastic cells; they also produce similar effects in normal cells. In this study, we hypothesized that the combination of idarubicin-bromelain could make cancer cells more susceptible to cytotoxicity and genotoxicity.Material...

The aim of this work was to isolate and purify bromelain extracted from the pineapple peel by ammonium sulfate precipitation (40-80%), followed by desalting and freeze-drying with a 75% activity recovery and 2.2 fold increased specific activity. Ion exchange chromatography on DEAE-Sepharose was able to separate the polysaccharides from the enzyme, which was recovered in the elution step, mainta...

Amaç: Metotreksat, bazı kanser türleri ve romatoid artrit başta olmak üzere çeşitli hastalıkların tedavisinde kullanılan bir folik asit antagonistidir. Ancak, metotreksat kullanımıyla birlikte görülen nörotoksisiteyi de içeren birçok yan etki önemli klinik problemdir. Ananas bitkisinden elde edilen bromelain, radikal süpürücü lipit peroksidasyonu önleyici etkileri olan enzimatik karışımdır. Ger...

Background: Fungal keratitis is an acute or chronic inflammation of the cornea caused by fungi. The main treatment for fungal Natamycin 5%. Disadvantages natamycin are its poor penetration and increased resistance due to fungi which lower level ergosterol in wall. Purpose: Investigates effect bromelain, zingibain papain on LOX-1 receptors Aspergillus fumigatus. Result: receptor with bromelain p...

The objective of this study was to determine the antimicrobial activity of proteolytic, meat-tenderizing enzymes (papain and bromelain) against E. coli and L. monocytogenes at three different temperatures (5, 25 and 35°C). Two overnight cultures of E. coli JM109 and L. monocytogenes were separately suspended in 1% peptone water and exposed to a proteolytic enzyme (papain or bromelain) at three ...

The purpose of this study was to investigate the application of bromelain in the treatment of pain, swelling, and limited mouth opening in patients with hematologic tumor after extraction of the third molar during chemotherapy. We recruited 72 patients with hematologic tumor who were treated by oral and maxillofacial surgery in our hospital, and third molar extraction was performed under local ...

Two forms of an acidic bromelain proteinase isolated from crude bromelain, an extract from pineapple stem, were found by a two-step FPLC purification procedure. The basic main components were removed by cation exchange chromatography and the breakthrough fraction was further resolved by anion exchange chromatography into 15 protein fractions, only two of which, called SBA/a and SBA/b, were prot...

Bromelain is a protease enzyme obtained from pineapple. In choosing bromelain only pay attention to its chemical analysis and there no rank yet. Therefore, research has been carried out in making the best decision using Multiple Attribute Decision Making with Simple Additive Weighting Method (MADM-SAW) method based on shelf life of pineapples. The basic concept this find weighted sum performanc...

Rheumatic diseases are traditionally treated using symptomatic drugs (NSAIDs Nonsteroidal Anti-Inflammatory Drugs) and specific drugs. Nevertheless, the inhibitory effect of traditional NSAIDs, caused by both types of COX enzymes, besides easing inflammation and pain, also has a number of damaging effects on gastric mucosa protection mechanisms. Therefore, NSAIDs administration is also contrain...