The Resistance to Powdery Mildew 8 (RPW8) locus confers broad-spectrum resistance to powdery mildew in Arabidopsis thaliana. There are four Homologous to RPW8s (BrHRs) in Brassica rapa and three in Brassica oleracea (BoHRs). Brassica napus (Bn) is derived from diploidization of a hybrid between B. rapa and B. oleracea, thus should have seven homologs of RPW8 (BnHRs). It is unclear whether these...

Chloroplast simple sequence repeats amplicons in 5 subspecies of Brassica oleracea were sequenced, and one chloroplast SNP was detected in amplicon ACP43. Through the introduction of an RsaI recognition site by adding one mismatch in the forward primer, combined with the increased primer length and raised annealing temperature, the dCAPS (derived cleaved amplified polymorphic sequences) marker ...

BACKGROUND AND AIMS Plants damaged by herbivores emit a variety of volatile organic compounds (VOCs). Here we used proton-transfer reaction mass spectrometry (PTR-MS) as a sensitive detection method for online analysis of herbivore-induced VOCs. Previously, it was found that Brassica nigra plants emit several sulfur-containing VOCs when attacked by cabbage root fly (Delia radicum) larvae with m...

We describe the construction of a BAC contig and identification of a minimal tiling path that encompass the dominant and monogenically inherited downy mildew resistance locus Pp523 of Brassica oleracea L. The selection of BAC clones for construction of the physical map was carried out by screening gridded BAC libraries with DNA overgo probes derived from both genetically mapped DNA markers flan...

chlormequat (cycocel or ccc), the plant growth retardant, was evaluated for its ability to control plant height in brassica oleracea cultivar ‘kamome white’ and ‘nagoya red’. different concentrations of ccc (0, 500, 1000 and 1500 mg/l) were sprayed and drenched on plants 40 days after transplanting. data were recorded the 60 and 90 days after transplanting. the 1500 mg/l of ccc resulted in abou...

Transposable elements (TEs) are the major component of plant genomes where they contribute significantly to the >1,000-fold genome size variation. To understand the dynamics of TE-mediated genome expansion, we have undertaken a comparative analysis of the TEs in two related organisms: the weed Arabidopsis thaliana (125 megabases) and Brassica oleracea ( approximately 600 megabases), a species w...

In the present work, novel nanotool called 'nano-embedded fungus' formed by impact of synergistic association of ZnO-nanorods and fungus Piriformospora indica DSM 11827, for growth of Brassica oleracea var. botrytis (Broccoli) is reported. ZnO-nanorods were synthesized by mechanical assisted thermal decomposition process and characterized by scanning electron microscopy (SEM) for morphology, X-...

Correlation coefficients based on relative concentrations of 13 glucosinolates in the edible parts of 30 culth'ars were determined. Brussels sprouts (Brassica oleracea L. gemmifera group), cauliOower (8. oleracea L. botlJ·tis group), and either marrow-stem or smooth-leafed kale (B, oleracea L. aceplzala group) had similar glucosinolate patterns based on significant correlations (P < 0.01). The ...

Frost resistant mutants of Brassica oleracea var. botrytis were investigated for the presence of CBF/DREB1 and COR15a gene products and induced frost resistance. Total RNA of clones was isolated after 3 h, 6 h, 24 h and 14 d acclimation at 4 °C and proteins and free proline were isolated after 14 d acclimation. cDNA was produced using RT-PCR and the first CBF gene in B. oleracea detected and di...

results the average percentage of wound healing in different groups on days 3, 6, 9 and 12 were as follows; 21.3%, 37.87%, 67.39% and 77.17% in control group; 24.98%, 34.21%, 70.74% and 88.55% in positive control group; and 32.35%, 61.27%, 94.53%, and 99.91%, in case group. a significant difference was observed between the control and case groups (p < 0.05). conclusions treatment with the studi...