The in vitro antimicrobial susceptibility of the anaerobic intestinal spirochete Brachyspira pilosicoli was investigated by an agar dilution method. Human (n = 123) and porcine (n = 16) isolates were susceptible to metronidazole, ceftriaxone, meropenem, tetracycline, moxifloxacin, and chloramphenicol; erythromycin and ciprofloxacin were not active. Resistance to amoxicillin and clindamycin vari...

A novel TaqMan 5-plex real-time PCR was developed for the identification of Brachyspira species in swine. B. hyodysenteriae, pilosicoli, and suanatina are identified using three hydrolysis probes targeting cpn60, while hampsonii is recognized by another nox specific probe. This highly sensitive assay easy to implement routine veterinary diagnostic laboratories enables rapid differentiation betw...

BACKGROUND The anaerobic spirochetes Brachyspira hyodysenteriae and Brachyspira pilosicoli cause diarrheal diseases in pigs. Their fastidious nature has hampered standardization of methods for antimicrobial susceptibility testing. For monitoring of antimicrobial susceptibility wild type cutoff values are needed to define where the wild type distribution of MICs ends and no approved cutoffs are ...