Gemini surfactants and their interactions with proteins have gained considerable scientific interest, especially when amyloidogenic proteins are taken into account. In this work, the influence of two selected dicationic (gemini) surfactants (3,3'-[1,8-(2,7-dioxaoctane)]bis(1-dodecylimidazolium) chloride and 3,3'-[1,12-(2,11-dioxadodecane)]bis(1-dodecylimidazolium) chloride) on two model protein...

A primary advantage of label-free detection methods over fluorescent measurements is its quantitative detection capability, since an absolute measure of adsorbed material facilitates kinetic characterization of biomolecular interactions. Interferometric techniques relate the optical phase to biomolecular layer density on the surface, but the conversion factor has not previously been accurately ...

Mass-sensitive electro-acoustic devices such as surface acoustic wave (SAW)micro-balances, capable to operate with aqueous media are particularly favorable for thedevelopment of biosensors. Their dimensions and physical properties offer a large potentialin biological fluid investigations, especially for measuring physical phenomenon (massdeposition, adsorption, pressure...). In this work, we pr...

Birefringence relaxation studies on bovine serum albumin (BSA) reveal transient decay described by a double exponential process. The values of the relaxation times lead to estimation of the size of the equivalent ellipsoid of revolution for BSA. Previous measurements of transient birefringence for BSA have shown a single relaxation process, since the apparatus used in obtaining those data was n...

In this work, a nanofluidic device containing both a feed cell and a permeation cell linked by nanopore arrays has been fabricated, which is employed to investigate thermally controlled biomolecular transporting properties through confined nanochannels. The ionic currents modulated by the translocations of goat antibody to human immunoglobulin G (IgG) or bovine serum albumin (BSA) are recorded ...

This paper reports on the study of the interactions between ascorbic acid (AA) and bovine serum albumin (BSA) in aqueous solution as well as in films (BSA/AA films) prepared by the layer-by-layer technique. Regarding to solution studies, a hyperchromism (in the range of ultraviolet) was found as a function of AA concentration, which suggested the formation of aggregates from AA and BSA. Binding...

A tetraphenylethene (TPE) group that exhibits aggregation-induced emission is incorporated into the ligand of a Eu(III) complex (TPEEu) to sensitize the excited state of Eu(III). In steady-state measurements, TPEEu exhibits weak luminescence when dissolved in aqueous solutions even at a high concentration level, but emits strong fluorescence of TPE and phosphorescence of Eu(III) upon binding wi...

An expeditious and accurate simplification of Stone and Scallan's solute exclusion technique was developed, thereby avoiding several sources of experimental error coupled with the determination of cellulose pore volume. Using this method, it is shown that cellulolytic enzymes do not enter into the micropores of five studied celluloses. These results suggestes that hydrolysis occurs initially at...

A method for solubilization of pristine fullerene (C(60)) in water was proposed by directly using bovine serum albumin (BSA) as a "solubilizer" by means of the unfolding mechanism. C(60) aqueous solution with a small distribution size, excellent dispersion stability and high dispersion concentration was obtained without the use of derivatization and organic solvent.

Conjugates of bovine serum albumin with progesterone, deoxycorticosterone, and estrone have now been synthesized as an extension of a program to prepare and characterize steroid-protein conjugates with the ultimate purpose of testing their ability to stimulate the formation of antihormonal antibodies (1). The steroid derivatives which were coupled to albumin are shown in Fig. 1. The synthetic a...