We present detailed quantitative measurement analyses for flow in a spinner flask with spinning rates between 20 to 45 RPM, utilizing the optical velocimetry measurement technique of Particle Image Velocimetry (PIV). A partial section of the impeller was immersed in the working fluid to reduce the shear forces induced on the cells cultured on microcarriers. Higher rotational speeds improved the...

To mimic in vivo vibration of vocal fold cells, we studied the controllability and range of frequency, acceleration, duration, and shear stress in a new bioreactor attachment. The custom multiwell disc appliance fits into a commercially built rheometer, together termed a torsional rheometer bioreactor (TRB). Previous attachments to the TRB were capable of 50–100 Hz vibrations at relatively high...

We describe a three-dimensional numerical simulation of interstitial flow through the medial layer of an artery accounting for the complex entrance condition associated with fenestral pores in the internal elastic lamina (IEL) to investigate the fluid mechanical environment around the smooth muscle cells (SMCs) right beneath the IEL. The IEL was modeled as an impermeable barrier to water flow e...

A separate-bottom Couette cell is used to carry out ring-shear tests on sand and hydrate, the evolution of shear bands recognized using tracer method. Based experimental verification, a numerical simulation method applied study band variation law with height strength sample. Analyzing distribution stress strain gives dimensionless number (c+ρgH·tgφ)H/αGL that affects characteristics band, which...

This paper presents an experimental research on the distribution of local friction factor, fb, and global friction factor, f, over the cross-section of a channel with V-shaped bottom, which typically occurs in sewers and culverts. Several series of experiments were conducted for measuring velocity and boundary shear stress. It is found that, Darcy-Weisbach, f, is more sensitive than other resis...

Mechanical forces are important regulators of cell biology in health and disease. Cells in the vascular system are subjected to fluid shear stress, cyclic stretch, and differential pressure [1]. Numerous investigations have revealed the vast pathological and physical responses of endothelial cells to fluid shear stress by culturing the cells on the rigid surfaces of a flow chamber. This app...

this study proposed a new discharge estimation method using a mean velocity formula derived from chiu’s 2d velocity formula of probabilistic entropy concept and the river bed shear stress of channel. in particular, we could calculate the mean velocity, which is hardly measurable in flooding natural rivers, in consideration of several factors reflecting basic hydraulic characteristics such as r...

Arsenic released by bottom sediments was determined by experiments in which the sediments were artificially re-suspended using a particle entrainment simulator (PES) to simulate river conditions. Sediment cores were collected from various tributaries to drinking water reservoirs in Connecticut spiked with arsenic, and run in the PES at simulated bed-flow shear stresses from 0.0 to 0.6 N/m(2). U...

Fluid shear stress alters the morphology and function of the endothelium by activating several kinases. Furthermore, shear stress potently inhibits apoptosis of endothelial cells. Since activation of Akt kinase has been shown to prevent cell death, we investigated the effects of shear stress on Akt phosphorylation. To test the hypothesis that shear stress interacts with the Akt kinase pathway, ...