a purification procedure of pertussis toxin (pt) from submerged culture of bordetella pertussis (b.pertussis) strain 134 using adsorption and affinity chromatography was discussed. the yield of the resulting pt was approximately 37.5mg/l of concentrated culture supernatant. the polypeptide pattern of the purified pt was investigated by sds-page and showed five bands between 11 to 26 kda using l...

this study was carried out to examine aflatoxin b1 (afb1) removal ability of four strains of lactic acidbacteria (lab). three indigenous (lactobacillus rhamnosus tmu094, lactobacillus fermentum tmu121and pedioccus pentosaceus tmu457) and a non-indigenous (labacillus rhamnosus ptcc1637) isolateswere studied. the strains were incubated with (afb1) at different time. the toxin residual in the supe...

Botulinum toxin (BoNT), being one of the most toxic substances known to mankind, due its unique mechanism action is used as an effective treatment for many diseases, including strabismus, blepharospasm, and hemifacial spasm. BoNT has received particular use a long-acting analgesic ability inhibit exocytosis pain neuropeptides, CGRP, Substance P, modulate expression pain-related receptors TRPV1,...

Rhizoctonia solani causes stem canker and significantly impacts potato production. Conventional assay to evaluate resistance using R. inoculum is time consuming. To establish an effective fast assay, 20 cultivars/lines were examined both AG2-1 as its derived toxin (RS toxin). In field trials of 2009 2010, wheat-bran-mediated was incorporated at either 0, 2, 3, 4 or 5 g per seed piece in the soi...

background: t-2 toxin is a mycotoxin of type a trichothecenes produced by several fungal genera such as fusarium species. mycotoxins can affect both cell mediated and humoral immune compartments. objective: the purpose of this study was to investi-gate the effect of t-2 toxin on cytokine production by mouse peritoneal macrophages and lymph node t cells. methods: mouse peritoneal macrophages and...

in this research a molecular method based on polymerase chain reaction for typing of clostridium perfringens was developed and toxin genotypes of 64 isolates from sheep and goats in iran were determined. the pcr assays were developed for detection of alpha (cpa), beta (cpb) and epsilon (etx) toxin genes, allowing classification of the isolates into genotypes a b, c and d. the field isolates ...

purpose : the aim of this study is to evaluate the effectiveness of intraoperative botulinum toxin-a (bta) injection as an adjunct to the surgical treatment of large-angle esotropia or exotropia. methods : thirteen patients were included in this interventional case series study. mean age of the patients was 23.31±13.29 years (range: 5-43 years). of these 13 patients, 6 were exotropic and 7 were...

background: achalasia is the most recognized esophageal motor disorder with an unknown etiology. symptoms rather than physical findings are hallmarks of the disease. modalilies for treatment include pharmacologic, botulinum toxin injection, pneumatic dilation and surgery. materials and methods: to review the diagnostic and therapeutic options currently available for achalasia. a medline search ...

Diphtheria Toxin Repressor (dtxR) Gene-Based Genetic Diversity of Corynebacterium diphtheriae Isolated in Jakarta, Indonesia, 2018–2019