Single-molecule spectroscopy (SMS) is a powerful experimental technique used to investigate a wide range of physical, chemical, and biophysical phenomena. The merit of SMS is that it does not require ensemble averaging, which is found in standard spectroscopic techniques. Thus SMS yields insight into complex fluctuation phenomena that cannot be observed using standard ensemble techniques. We in...

We recorded fluorescence time traces, and simultaneously either the fluorescence lifetime or the emission spectra from single perylene bisimide (PBI) dimers embedded in a polystyrene matrix. In these traces three distinct intensity levels can be distinguished, which reflect the photo-induced radicalisation of one of the perylene subunits. Differences in the energy transfer rate between the neut...

The mechanisms of tear flow, blinking, and tear drainage were studied employing high speed slit-lamp cinematography using a polystyrene microsphere suspension as a tracer of tear movement. It was demonstrated that: (1) Tears are drawn into the canaliculi during the relaxation phase of blinking. (2) Both superior and inferior puncta function as normal tear drainage routes. (3) The superior punct...

We investigate the dynamics of N moving point vortices, whose vorticity changes periodically between a finite value and zero. The dynamics of such blinking vortices is chaotic, but the degree of chaoticity, expressed in terms of finite size Lyapunov exponents, decreases monotonically with time. In contrast to traditional point vortices, the average diameter of the blinking vortex system increas...

Direct numerical simulations of chevrons, blinking states, and repeated transients in binary fluid mixtures with a negative separation ratio heated from below are described. The calculations are performed in two-dimensional containers for experimental parameter values and boundary conditions. Quantitative agreement with the experiments of Kolodner [Phys. Rev. E 47, 1038 (1993)] is obtained, and...

The fluorescence intermittency of multilayered quantum dots QDs is experimentally investigated. The measured blinking statistical probabilities for QDs of different shell structures and at different excitation powers are compared with the diffusion controlled electron transfer model. The results show that the power law statistics for the “on” and “off” events depend strongly on the structure an...

The cornea has been a focus of animal electrophysiological research for decades, but little is known regarding its cortical representation in the human brain. This study attempts to localize the somatotopic representation of the cornea to painful stimuli in human primary somatosensory cortex using functional magnetic resonance imaging (fMRI). In this case study, a subject was imaged at 3T while...

In this paper, a new acquisition protocol is adopted for identifying individuals from electroencephalogram signals based on eye blinking waveforms. For this purpose, a database of 10 subjects is collected using Neurosky Mindwave headset. Then, the eye blinking signal is extracted from brain wave recordings and used for the identification task. The feature extraction stage includes fitting the e...