Scoring matrices for nucleic acid sequence comparison that are based on models appropriate to the analysis of molecular sequencing errors or biological mutation processes are presented. In mammalian genomes, transition mutations occur significantly more frequently than transversions, and the optimal scoring of sequence alignments based on this substitution model differs from that derived assumi...

Three alkaline protease producing gram positive, spore forming Streptomyces (J1, J2 and J3) were isolated from alkaline soil (pH range: 9-10) samples collected from Central Soil Salinity Research Institute (C.S.S.R.I), Lucknow. On the basis of halo zone, morphology, texture and microscopic characteristics they were identified as Streptomyces. Further characterization both genetically and phenot...

MOTIVATION Homology search finds similar segments between two biological sequences, such as DNA or protein sequences. The introduction of optimal spaced seeds in PatternHunter has increased both the sensitivity and the speed of homology search, and it has been adopted by many alignment programs such as BLAST. With the further improvement provided by multiple spaced seeds in PatternHunterII, Smi...

Differential display gels were run for the drought tolerant (N-22) and drought susceptible (Panidhan) genotypes of rice (Oryza sativa) to identify the genes showing differential expression with respect to moisture stress. Differential cDNA products were cloned in PCR-Trap vector and analyzed for differential expression by Northern hybridization. Two clones namely R4A and R7G were found to be as...

Soybean rust is caused by the obligate fungal pathogen Phakopsora pachyrhizi Sydow. A unidirectional cDNA library was constructed using mRNA isolated from germinating P. pachyrhizi urediniospores to identify genes expressed at this physiological stage. Single pass sequence analysis of 908 clones revealed 488 unique expressed sequence tags (ESTs, unigenes) of which 107 appeared as multiple copie...

The mtDNA sequences revealed that several of the fish studied were Hampala macrolepidota and Barbonymus gonionotus . objective this research was to learn pattern COI gene in establish a phylogenetic tree. Basic Local Alignment Search Tool-nucleotide (BLASTn) confirmed froma Ranau Lake, South Sumatera has 100% matching ranges species from Memberamo River (Indonesia), India, Bangladesh, Thailand ...

Aim. The aim of the work is to establish level similarity primary structure and organization landomycin clusters streptomycetes. Methods. Information on nucleotide sequences annotations streptomycetic biosynthesis genes AF080235,1, KJ645792.1, HQ828984.1 genomes strains S. cyanogenus S136, globisporus 1912-4Crt freely available in GenBank. Analysis streptomycetes was performed using program BLA...

This is a first molecular report investigate the phylogenic analysis of Theileria spp. in sheep and it's infested ticks Mosul city-Iraq. A total 185 blood samples were collected from different areas city. sixty five Ixodid also parts infected animals. The overall prevalence was 52.4% (97 out 185), for ovis it 9.7%, lestoquardi 20% mixed infection 22.7% city, based on conventional-PCR multiplex-...

Aim. The goal is to determine the similarities and differences in primary structure of genes that chitinases from GH19 family strains S. griseus HUT6037 coelicolor A3(2). Investigate distribution streptomycetes genomes sequences are similar сhiC сhiF genes. Methods. Information on nucleotide annotations studied streptomycete chromosomes (including chitinase A3(2) HUT6037) freely available GenBa...

BACKGROUND Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide databases. Althoug...