Blackleg disease, caused by the fungal pathogen Leptosphaeria maculans, is most devastating disease of canola (Brassica napus, oilseed rape) worldwide. Breeding for genetic resistance widely used tool controlling this and minimizing impact on yield. To date, five genes (Rlm2, LepR3, Rlm4, Rlm7, Rlm9) have been cloned from B. representing alleles two different gene loci, Rlm2-LepR3 Rlm4-7-9. We ...

Improved conventional PCR techniques are required for the rapid on-site detection of human and animal diseases. In this context, a PCR method using dry-stored reagents intended for the detection of Clostridium spp. is presented. Basic PCR reagents (BSA, PCR buffer, MgCl₂ and primers), which were dried on polyolefin matrices, showed stability at ambient temperatures for up to 10 months without a...

The distribution of pathogenic anaerobes in the environment and the relationship with diseases in animals are discussed. A distinction between the spore-bearing anaerobes (clostridia) and the Gram-negative non-spore-forming anaerobes is necessary. The main habitat of clostridia is the soil but they are also found in dust, sewage, rivers, lakes, sea water, milk, vegetables, fresh meat, fish, ins...

The most important aerobic bacterial diseases of farmed deer and bison include bovine tuberculosis, Johne's disease (paratuberculosis), yersiniosis, leptospirosis, brucellosis, pasteurellosis, anthrax, salmonellosis and colibacillosis. Anaerobic bacterial infections affecting the same animals include necrobacillosis and a number of clostridial diseases such as tetanus, blackleg, malignant oedem...