AIM The aims of this study were to investigate the phenotypic and genotypic of extended-spectrum β-lactamase (ESBL) and metallo-β-lactamases (MBLs) and determine phylogenetic background E. coli isolates from fecal samples of patients with diarrhea in Kerman, southeast of Iran. BACKGROUND The emergence of ESBLs and MBLs-producing E. coli caused problems in antibiotic treatments. E. coli strain...

OBJECTIVE To investigate the local epidemiology of Klebsiella penumoniae carrying bla(CTX-M-15) in southern China and to characterize the genetic environment of bla(CTX-M-15). METHODS PCR and DNA sequencing were used to detect and characterize the genetic contexts of bla(CTX-M-15). The clonal relatedness of isolates carrying bla(CTX-M-15) was determined by pulse-field gel electrophoresis. Con...

We report the case of a pediatric patient with a Salmonella enterica serotype Infantis infection. Detailed microbiological investigation revealed that this isolate carries four beta-lactamase genes (bla(TEM-1b) variant, bla(SHV-5), bla(CTX-M-15), and bla(CMY-2)) conferring resistance to all beta-lactams but imipenem. This is the first report of a Salmonella isolate with CTX-M and AmpC enzymes o...

BACKGROUND Penicillinase-producing Neisseria gonorroheae (PPNG) was first isolated in 1976. PPNG strains carrying bla TEM-1 and bla TEM-135 gene have been described in different countries. Recently, a novel bla TEM-220 allele was detected in PPNG isolates carrying Toronto/Rio plasmid. The prevalence and characteristics of TEM-220 strains worldwide are unknown, and therefore, it needs to be stud...

OBJECTIVES To study the evolution between 1999 and 2002 and mechanisms of antibiotic resistance in a multidrug-resistant Acinetobacter baumannii clone predominant in isolates from elderly patients with respiratory tract infections. METHODS Susceptibility to antimicrobials was determined using an agar dilution method. Bacterial clones were identified by PCR-fingerprinting and PFGE with ApaI. C...

OBJECTIVES This study was designed to demonstrate the prevalence of the newly discovered carbapenem-hydrolysing class D enzymes, OXA-51-type and OXA-58, among clinical isolates of Acinetobacter spp. METHODS A total of 72 isolates from six centres were studied. Isolates were screened by PCR with specific primers for bla(OXA-51-type) and bla(OXA-58). PCR products were sequence-analysed. Plasmid...

BACKGROUND KPC carbapenemase-producing Klebsiella pneumoniae isolates have been increasingly detected in Europe. We report the first KPC-producing isolates characterized in Madrid, Spain. METHODS Twelve K. pneumoniae isolates recovered from clinical and surveillance cultures from eight patients (September 2009 to February 2010) that were resistant to carbapenems and resulted in a positive mod...

BACKGROUND Antibiotic resistance is widespread among diarrheagenic Escherichia coli in developing countries, where the overuse of antibiotics is common. Information regarding β-lactamases, especially Extended-Spectrum β-Lactamases (ESBLs) in diarrheagenic pathogens should be considered in clinical management when an optimal treatment is needed. OBJECTIVES The main objective of this study was ...

We previously found that long-term potentiation (LTP) in the dentate gyrus (DG) is attenuated by lesion of the basolateral amygdala (BLA), but it remained unclear whether or not there is neural connection between the BLA and the DG. In the present study, we tried to provide physiological evidence that the DG receives neural inputs from the BLA. Single-pulse electrical stimulation of the BLA evo...

OBJECTIVES Linked bla(CTX-M-3)-bla(TEM-1b) genes, as found on the IncI1 plasmid pEK204 prevalent in Belfast, also occur on plasmids belonging to other rep types in Escherichia coli isolated from nursing-home residents in Belfast. We investigated the mechanisms for their joint dissemination among diverse plasmids. METHODS Plasmid pEK204 was transferred by electroporation into E. coli DH5α harb...