The introduction of genetic material into cells and tissues of interest remains a rate-limiting step for molecular investigations in many fields, especially neurobiology. Many different methods have been developed to transfect cells—DNA-mediated gene transfer, microinjection, electroporation, lipofection, and viral transfection—and for many cell types, gene transfer using these methods has beco...

Bacterial genes responsible for poly(3-hydroxybutyrate) (PHB) biosynthesis were targeted to plant peroxisomes by adding a carboxy-terminal targeting sequence. The enzymes evidently were transported into peroxisomes, retained their catalytic activity, and reacted with peroxisomally available precursors because PHB synthesis in transgenic plant cells was localized to peroxisomes. Up to 2 mg/g fre...

Genetic improvement of soybean seed traits is important for developing new varieties that meet the demand as a food, forage crop, and industrial products. A large number genome sequences are currently publicly available. This sequence information provides significant opportunity to design genomic approaches improve traits. Genome editing represents major advancement in biotechnology. The produc...